{"id":"986c7082-af62-5049-9d26-7995d352448c","stable_key":"e0801c92-7fd3-5cb2-82b7-dd82f010f3ed:ivermectin-leukemia-chloride-influx","predicate":"increases","statement":"Ivermectin increased intracellular chloride concentration and cell size in leukemia cells, accompanied by plasma membrane hyperpolarisation without a change in mitochondrial membrane potential.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ed5b57c6-e0ab-5392-b0b2-e8b411d133c9","mechanism_event_label":"Ivermectin increased intracellular chloride concentration and cell size in leukemia cells, accompanied by plasma membrane hyperpolarisation without a change in mitochondrial membrane potential.","subject":{"id":"416b9886-c6a5-5526-a8bf-b571a8d418bf","slug":"ivermectin","display_name":"Ivermectin","entity_type_key":"drug"},"object":{"id":"8be23eb0-6c40-5646-bdac-b802a442b9d9","slug":"leukemia-intracellular-chloride","display_name":"Intracellular chloride concentration in leukemia cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ed5b57c6-e0ab-5392-b0b2-e8b411d133c9","stable_key":"e0801c92-7fd3-5cb2-82b7-dd82f010f3ed:ivermectin-leukemia-chloride-influx-event","event_type":"observed_relationship","label":"Ivermectin increased intracellular chloride concentration and cell size in leukemia cells, accompanied by plasma membrane hyperpolarisation without a change in mitochondrial membrane potential.","description":"Ivermectin increased intracellular chloride concentration and cell size in leukemia cells, accompanied by plasma membrane hyperpolarisation without a change in mitochondrial membrane potential.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"416b9886-c6a5-5526-a8bf-b571a8d418bf","slug":"ivermectin","display_name":"Ivermectin","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8be23eb0-6c40-5646-bdac-b802a442b9d9","slug":"leukemia-intracellular-chloride","display_name":"Intracellular chloride concentration in leukemia cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6417a8f5-5da0-51ac-88e3-f7f0b6567498","slug":"leukemia-plasma-membrane-potential","display_name":"Plasma membrane potential of leukemia cells","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Acute myeloid leukemia cell lines and primary patient samples","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Low micromolar ivermectin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The mitochondrial membrane potential was explicitly unchanged, which separates this from mechanisms that act through mitochondrial depolarisation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Acute myeloid leukemia cell lines and primary patient samples","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Ivermectin increased intracellular chloride concentration and cell size in leukemia cells, accompanied by plasma membrane hyperpolarisation without a change in mitochondrial membrane potential.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The antiparasitic agent ivermectin induces chloride-dependent membrane hyperpolarization and cell death in leukemia cells. (2010). https://pubmed.ncbi.nlm.nih.gov/20644115/ DOI: 10.1182/blood-2010-01-262675","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Chloride flux and membrane potential","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a670204c-7f1d-530c-8294-0477d19fd3fb","evidence_kind":"source_excerpt","locator":"Lines 178-187","start_line":178,"end_line":187,"excerpt":"## ivermectin-leukemia-chloride-influx\nIvermectin increased intracellular chloride concentration and cell size in leukemia cells, accompanied by plasma membrane hyperpolarisation without a change in mitochondrial membrane potential.\nModel/species: Acute myeloid leukemia cell lines and primary patient samples\nTissue/system: Chloride flux and membrane potential\nExposure: Low micromolar ivermectin\nRoute: In vitro\nDuration: Not stated here\nLimits: The mitochondrial membrane potential was explicitly unchanged, which separates this from mechanisms that act through mitochondrial depolarisation.\nPrimary reference: The antiparasitic agent ivermectin induces chloride-dependent membrane hyperpolarization and cell death in leukemia cells. (2010). https://pubmed.ncbi.nlm.nih.gov/20644115/ DOI: 10.1182/blood-2010-01-262675\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"20818ddd-7b37-5226-a24b-7de007e80eb2","stable_key":"import-e0801c92-7fd3-5cb2-82b7-dd82f010f3ed","title":"Ivermectin: mechanism of action across parasite, host barrier and mammalian targets (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of sixteen primary studies resolved by PubMed title search and cross-checked against live PubMed metadata. Study-specific citations, concentrations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"c7e1e0201cbd222f0c52b7981f078936ed39b617d747821ed6daa584c2b964a7","revision_id":"56746c3e-cdec-5b8f-a191-425de1a0a7ce","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}