{"id":"9813261c-63be-593e-b59e-43e0504f2c59","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-gpx4-pl-hydroperoxide-reduction","predicate":"reduces","statement":"Purified PHGPX (GPX4) with glutathione reduced phospholipid hydroperoxides within photooxidized human erythrocyte ghost membranes to alcohol products without prior phospholipase cleavage.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"34ef4eaf-4a71-575e-a00a-01436251816e","mechanism_event_label":"GPX4 removed peroxide groups from membrane phospholipids using glutathione.","subject":{"id":"e9bb13ee-f238-51e6-9101-78ddfd11465c","slug":"gpx4","display_name":"GPX4","entity_type_key":"protein"},"object":{"id":"5b200520-e015-50ee-8fbf-e0e0707296f1","slug":"phospholipid-hydroperoxides","display_name":"Phospholipid hydroperoxides","entity_type_key":"lipid"},"evidence_count":1,"mechanism_event":{"id":"34ef4eaf-4a71-575e-a00a-01436251816e","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-gpx4-pl-hydroperoxide-reduction-event","event_type":"biochemical_relationship","label":"GPX4 removed peroxide groups from membrane phospholipids using glutathione.","description":"Purified PHGPX (GPX4) with glutathione reduced phospholipid hydroperoxides within photooxidized human erythrocyte ghost membranes to alcohol products without prior phospholipase cleavage.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"reducing cosubstrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"37c8cd50-9ecb-572c-9de6-1c3be7f2522a","slug":"phospholipid-alcohols","display_name":"Hydroxy phospholipids","entity_type_key":"lipid"},"role":"products","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e9bb13ee-f238-51e6-9101-78ddfd11465c","slug":"gpx4","display_name":"GPX4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5b200520-e015-50ee-8fbf-e0e0707296f1","slug":"phospholipid-hydroperoxides","display_name":"Phospholipid hydroperoxides","entity_type_key":"lipid"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rose-bengal photoperoxidation followed by enzyme treatment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"GSH/PHGPX after photooxidation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified-enzyme preparation; distinguishes peroxide removal from vitamin E radical trapping.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human-derived cell-free membranes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"GPX4 removed peroxide groups from membrane phospholipids using glutathione.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ver-thomas1990] Protective action of phospholipid hydroperoxide glutathione peroxidase against membrane-damaging lipid peroxidation. In situ reduction of phospholipid and cholesterol hydroperoxides. (1990). https://pubmed.ncbi.nlm.nih.gov/2294113/ DOI: 10.1016/s0021-9258(19)40252-4","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Erythrocyte ghosts","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c67b2c03-647e-507c-b556-28ff25491f2a","evidence_kind":"source_excerpt","locator":"Lines 550-562","start_line":550,"end_line":562,"excerpt":"### ver-gpx4-pl-hydroperoxide-reduction\nPurified PHGPX (GPX4) with glutathione reduced phospholipid hydroperoxides within photooxidized human erythrocyte ghost membranes to alcohol products without prior phospholipase cleavage.\nCondition category: normal\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: GPX4 removed peroxide groups from membrane phospholipids using glutathione.\norganism: Human-derived cell-free membranes\ntissue_or_cell_type: Erythrocyte ghosts\nexperimental_model: Rose-bengal photoperoxidation followed by enzyme treatment\nlimitations: Purified-enzyme preparation; distinguishes peroxide removal from vitamin E radical trapping.\nexposure: GSH/PHGPX after photooxidation.\ncross_nutrient: true\nevidence_location: Primary abstract\n[ver-thomas1990] Protective action of phospholipid hydroperoxide glutathione peroxidase against membrane-damaging lipid peroxidation. In situ reduction of phospholipid and cholesterol hydroperoxides. (1990). https://pubmed.ncbi.nlm.nih.gov/2294113/ DOI: 10.1016/s0021-9258(19)40252-4","model_system":"Rose-bengal photoperoxidation followed by enzyme treatment","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ver-thomas1990] Protective action of phospholipid hydroperoxide glutathione peroxidase against membrane-damaging lipid peroxidation. In situ reduction of phospholipid and cholesterol hydroperoxides. (1990). https://pubmed.ncbi.nlm.nih.gov/2294113/ DOI: 10.1016/s0021-9258(19)40252-4","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3ac41dba-4727-5116-a556-ac3bbb0dc41c","stable_key":"import-e0ea2d6a-7429-5e9f-b774-41e5e3288da3","title":"Vitamin E: transport, membrane protection and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a90b6565e092c6a2da07f74cf758792c2cf63b40746260c8ff12bd64322c36f4","revision_id":"956793d1-23e4-5397-9291-25c1732a8e02","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}