{"id":"980b908e-2000-5de5-8d77-e1530b0b43c2","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-tfeb-translation","predicate":"hypusination_supports_translation","statement":"Disrupting eIF5A hypusination reduced nascent TFEB synthesis in the B-cell/autophagy study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2060c9f6-aca0-5424-9400-a7c3a83a3e3f","mechanism_event_label":"Spermidine helps produce a regulator of cellular recycling.","subject":{"id":"d220a16a-9534-52fd-a43c-cc577f7bc4aa","slug":"mouse-eif5a","display_name":"Mouse eIF5A translation factor","entity_type_key":"protein"},"object":{"id":"bdd7a685-e772-55c0-a183-bc0bdccb61cc","slug":"mouse-tfeb","display_name":"Mouse transcription factor EB / Tfeb","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"2060c9f6-aca0-5424-9400-a7c3a83a3e3f","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-tfeb-translation-event","event_type":"observed_relationship","label":"Spermidine helps produce a regulator of cellular recycling.","description":"Disrupting eIF5A hypusination reduced nascent TFEB synthesis in the B-cell/autophagy study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d220a16a-9534-52fd-a43c-cc577f7bc4aa","slug":"mouse-eif5a","display_name":"Mouse eIF5A translation factor","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bdd7a685-e772-55c0-a183-bc0bdccb61cc","slug":"mouse-tfeb","display_name":"Mouse transcription factor EB / Tfeb","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e1bf3e36-5f3d-5e65-b356-cee03e8ec175","slug":"mouse-dhps","display_name":"Mouse deoxyhypusine synthase / Dhps","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse cellular experiments with translation labeling and hypusination perturbation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Requirement is transcript- and context-dependent, not equal enhancement of all proteins.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Spermidine helps produce a regulator of cellular recycling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31474573/ · DOI 10.1016/j.molcel.2019.08.005","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fdd77df5-470f-5469-8e53-aef8cc279bbe","evidence_kind":"source_excerpt","locator":"Lines 214-220","start_line":214,"end_line":220,"excerpt":"## spermidine-tfeb-translation\nSpermidine helps produce a regulator of cellular recycling.\nDisrupting eIF5A hypusination reduced nascent TFEB synthesis in the B-cell/autophagy study.\nModel: Mouse cellular experiments with translation labeling and hypusination perturbation.\nLimitations: Requirement is transcript- and context-dependent, not equal enhancement of all proteins.\nEvidence access: Primary full text\nPolyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31474573/ · DOI 10.1016/j.molcel.2019.08.005","model_system":"Mouse cellular experiments with translation labeling and hypusination perturbation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8eec8784bc2bd0384cfd53c6603c4ced8e53c227d9ab29ed85594c43e34c68ef","revision_id":"7c421a18-4526-59f6-b84e-4be533bc35ac","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}