{"id":"97b8a58e-453e-558e-9ac5-781fb28f7f27","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-nad-calcium-response","predicate":"inhibits","statement":"Boric acid partly inhibited NAD+-evoked calcium transients at 250 µM and completely at 1,000 µM in DU-145 cells; NADP+-evoked and mechanical responses were inhibited at 1,000 µM.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"d5b0cdf8-2230-5121-b855-91fb324c7d7b","mechanism_event_label":"At the tested concentrations, boric acid reduced several ways these cancer cells released stored calcium.","subject":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"object":{"id":"a9f15f93-b978-5e9c-b3e3-dbf1134ae40a","slug":"intracellular-calcium-release","display_name":"Intracellular calcium-store release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d5b0cdf8-2230-5121-b855-91fb324c7d7b","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-nad-calcium-response-event","event_type":"biochemical_relationship","label":"At the tested concentrations, boric acid reduced several ways these cancer cells released stored calcium.","description":"Boric acid partly inhibited NAD+-evoked calcium transients at 250 µM and completely at 1,000 µM in DU-145 cells; NADP+-evoked and mechanical responses were inhibited at 1,000 µM.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"stimulus","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"be6e4670-953f-5a4e-97da-1e536a54e0e4","slug":"nadp-plus","display_name":"NADP+","entity_type_key":"small_molecule"},"role":"stimulus","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a9f15f93-b978-5e9c-b3e3-dbf1134ae40a","slug":"intracellular-calcium-release","display_name":"Intracellular calcium-store release","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/boron-research/18516691.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5fc4f02e11634c2a2d111a99ee4c6175fe8839a8f28152b656b3c29be6745206\", \"start_char\": 0, \"end_char\": 1733, \"text_sha256\": \"5fc4f02e11634c2a2d111a99ee4c6175fe8839a8f28152b656b3c29be6745206\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human prostate cancer cell culture and mass spectrometry","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Boric acid 100–1,000 µM; methylboronic acid comparator","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"These are cell-culture exposures, including concentrations far above usual circulating levels. Adduct detection and calcium responses do not prove cancer prevention or direct channel binding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Boron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"boron","display_name":"Boron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human DU-145 cells; cell-free complex analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"At the tested concentrations, boric acid reduced several ways these cancer cells released stored calcium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[boron-p18516691] Boric acid inhibits stored Ca2+ release in DU-145 prostate cancer cells. (2009). https://pubmed.ncbi.nlm.nih.gov/18516691/ DOI: 10.1007/s10565-008-9085-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Prostate cancer cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"369a8de3-03e0-5094-bdf0-27f73199d6ed","evidence_kind":"source_excerpt","locator":"Lines 274-285","start_line":274,"end_line":285,"excerpt":"### boron-nad-calcium-response\nBoric acid partly inhibited NAD+-evoked calcium transients at 250 µM and completely at 1,000 µM in DU-145 cells; NADP+-evoked and mechanical responses were inhibited at 1,000 µM.\nCondition category: normal\nnutrient_topic: Boron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: At the tested concentrations, boric acid reduced several ways these cancer cells released stored calcium.\norganism: Human DU-145 cells; cell-free complex analysis\ntissue_or_cell_type: Prostate cancer cells\nexperimental_model: Human prostate cancer cell culture and mass spectrometry\nlimitations: These are cell-culture exposures, including concentrations far above usual circulating levels. Adduct detection and calcium responses do not prove cancer prevention or direct channel binding.\nexposure: Boric acid 100–1,000 µM; methylboronic acid comparator\nevidence_span: {\"source_cache\": \"artifacts/boron-research/18516691.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5fc4f02e11634c2a2d111a99ee4c6175fe8839a8f28152b656b3c29be6745206\", \"start_char\": 0, \"end_char\": 1733, \"text_sha256\": \"5fc4f02e11634c2a2d111a99ee4c6175fe8839a8f28152b656b3c29be6745206\"}\n[boron-p18516691] Boric acid inhibits stored Ca2+ release in DU-145 prostate cancer cells. (2009). https://pubmed.ncbi.nlm.nih.gov/18516691/ DOI: 10.1007/s10565-008-9085-7","model_system":"Human prostate cancer cell culture and mass spectrometry","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [boron-p18516691] Boric acid inhibits stored Ca2+ release in DU-145 prostate cancer cells. (2009). https://pubmed.ncbi.nlm.nih.gov/18516691/ DOI: 10.1007/s10565-008-9085-7","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2d96c547-aeec-5d8b-9d31-1288da209f89","stable_key":"import-7edf94bb-95c8-5234-9161-9eb338bb9b36","title":"Boron: chemistry, nutrient interactions, low-intake studies and mechanistic uncertainties (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"0a32c1c5c70cfb4a378f6ace35cf5b75d90ed3065ada91b9f9f64015d603c8e3","revision_id":"38f316f2-4344-542e-a638-0ac5402f678d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}