{"id":"97a5c1e5-5a73-521d-a231-2609290a5c7d","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-pump-sodium","predicate":"reduces_vanadate_binding","statement":"Sodium decreased vanadate binding in magnesium-containing medium, whereas sodium and potassium had little effect in the manganese condition.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5650f0ba-f474-5814-b5fc-56785be5de27","mechanism_event_label":"The ionic environment changes how vanadate interacts with the pump.","subject":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"object":{"id":"4d1e42e4-30e4-53aa-b967-9a8e2ed3043a","slug":"dog-kidney-na-k-atpase","display_name":"Dog kidney sodium/potassium ATPase preparation","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"5650f0ba-f474-5814-b5fc-56785be5de27","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-pump-sodium-event","event_type":"observed_relationship","label":"The ionic environment changes how vanadate interacts with the pump.","description":"Sodium decreased vanadate binding in magnesium-containing medium, whereas sodium and potassium had little effect in the manganese condition.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4d1e42e4-30e4-53aa-b967-9a8e2ed3043a","slug":"dog-kidney-na-k-atpase","display_name":"Dog kidney sodium/potassium ATPase preparation","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Dog kidney enzyme binding assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No human dietary sodium recommendation follows.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"The ionic environment changes how vanadate interacts with the pump.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Vanadate binding to the (Na + K)-ATPase. · 1981 · https://pubmed.ncbi.nlm.nih.gov/6277881/ · DOI 10.1007/BF00743200","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a4df4138-d26c-55df-8fb2-a0bbb71d8424","evidence_kind":"source_excerpt","locator":"Lines 246-252","start_line":246,"end_line":252,"excerpt":"## vanadium-pump-sodium\nThe ionic environment changes how vanadate interacts with the pump.\nSodium decreased vanadate binding in magnesium-containing medium, whereas sodium and potassium had little effect in the manganese condition.\nModel: Dog kidney enzyme binding assay.\nLimitations: No human dietary sodium recommendation follows.\nEvidence access: Primary abstract\nVanadate binding to the (Na + K)-ATPase. · 1981 · https://pubmed.ncbi.nlm.nih.gov/6277881/ · DOI 10.1007/BF00743200","model_system":"Dog kidney enzyme binding assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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Not publisher full text.","file_path":"","sha256":"d36b30d972e756ca4d19b66f976980e206c65025bd4bbf4c127c17320bd019c2","revision_id":"70d42967-8032-5627-8670-f59841735e89","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}