{"id":"978c59df-936b-5143-a052-9f214237e094","stable_key":"05a3cf47-e660-5ab3-8c32-5eba9847cf0a:thc-anandamide-inhibits-cyclase","predicate":"inhibits","statement":"Anandamide interacted specifically with cannabinoid receptors and inhibited adenylate cyclase, reproducing the signature response of the plant cannabinoids.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"528d1092-6324-514a-9962-7bfe33e4200c","mechanism_event_label":"The natural ligand does the same thing to the same enzyme as the drug.","subject":{"id":"daf9c6ee-5fd9-529c-8dfb-f26606ae40e3","slug":"anandamide","display_name":"Anandamide / arachidonylethanolamide","entity_type_key":"small_molecule"},"object":{"id":"568c3837-1085-5594-bd46-6557c5e6fc73","slug":"adenylyl-cyclase-activity","display_name":"Adenylyl cyclase catalytic activity, model specified","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"528d1092-6324-514a-9962-7bfe33e4200c","stable_key":"05a3cf47-e660-5ab3-8c32-5eba9847cf0a:thc-anandamide-inhibits-cyclase-event","event_type":"biochemical_relationship","label":"The natural ligand does the same thing to the same enzyme as the drug.","description":"Anandamide interacted specifically with cannabinoid receptors and inhibited adenylate cyclase, reproducing the signature response of the plant cannabinoids.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d092243c-94e9-5f03-a406-2fa245f2f4ae","slug":"cnr1","display_name":"Human cannabinoid receptor 1 / CB1 / CNR1","entity_type_key":"protein"},"role":"target_receptor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"daf9c6ee-5fd9-529c-8dfb-f26606ae40e3","slug":"anandamide","display_name":"Anandamide / arachidonylethanolamide","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"568c3837-1085-5594-bd46-6557c5e6fc73","slug":"adenylyl-cyclase-activity","display_name":"Adenylyl cyclase catalytic activity, model specified","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/thc-research/8515284.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"36a5b1eec5097dbdfd0224629f4d8449f8688d0476820823ffcbe3b92d68c66d\", \"start_char\": 0, \"end_char\": 1015, \"text_sha256\": \"36a5b1eec5097dbdfd0224629f4d8449f8688d0476820823ffcbe3b92d68c66d\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Receptor binding and adenylate cyclase assays with anandamide","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Anandamide against cannabinoid receptors and cyclase","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Confirms the endogenous ligand reproduces the receptor signature of the drug.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"THC research collection; topical membership is not evidence of a direct clinical effect, and THC is recorded separately from the endocannabinoids it imitates.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tetrahydrocannabinol","display_name":"Delta-9-tetrahydrocannabinol / THC","entity_type_key":"drug"}},{"dimension":"organism","value_text":"Rat brain preparations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The natural ligand does the same thing to the same enzyme as the drug.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[thc-p8515284] Anandamide, a brain endogenous compound, interacts specifically with cannabinoid receptors and inhibits adenylate cyclase. 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(1993). https://pubmed.ncbi.nlm.nih.gov/8515284/ DOI: 10.1111/j.1471-4159.1993.tb03576.x","model_system":"Receptor binding and adenylate cyclase assays with anandamide","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [thc-p8515284] Anandamide, a brain endogenous compound, interacts specifically with cannabinoid receptors and inhibits adenylate cyclase. 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