{"id":"9772bde2-aa8f-55c4-9b3a-a9edcbec5181","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-g6pd-reduction-failure","predicate":"defect_limits","statement":"Erythrocytes from a G6PD-deficient person failed to reduce lipoic acid in the assay; pharmacological G6PD inhibition also prevented reduction.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e78e8fb3-4146-59af-8208-695f580b4c7a","mechanism_event_label":"Providing the oxidized molecule did not overcome the missing reducing capacity.","subject":{"id":"f65184d1-6b79-5e38-8137-a06a40e94e36","slug":"g6pd","display_name":"Glucose-6-phosphate dehydrogenase / G6PD","entity_type_key":"protein"},"object":{"id":"39c21335-a4c4-52d7-acb5-464ae9569bff","slug":"erythrocyte-free-lipoate-reduction","display_name":"Erythrocyte reduction of free lipoic acid","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e78e8fb3-4146-59af-8208-695f580b4c7a","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-g6pd-reduction-failure-event","event_type":"biochemical_relationship","label":"Providing the oxidized molecule did not overcome the missing reducing capacity.","description":"Erythrocytes from a G6PD-deficient person failed to reduce lipoic acid in the assay; pharmacological G6PD inhibition also prevented reduction.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ab091982-3acb-5a87-80fb-85dfe292c1e0","slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"440768d2-007b-5a78-b340-889759fbdb05","slug":"dihydrolipoic-acid","display_name":"Free dihydrolipoic acid / DHLA","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4aba2a5e-8d06-5304-bb01-c0402b225a94","slug":"nadph","display_name":"NADPH","entity_type_key":"small_molecule"},"role":"reducing_equivalent","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f65184d1-6b79-5e38-8137-a06a40e94e36","slug":"g6pd","display_name":"Glucose-6-phosphate dehydrogenase / G6PD","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"39c21335-a4c4-52d7-acb5-464ae9569bff","slug":"erythrocyte-free-lipoate-reduction","display_name":"Erythrocyte reduction of free lipoic acid","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/ala-research/7632170.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"2ebbc851e5279e646bc7c892645c931a3fd2993830c3f4405379839cbbd6e707\", \"start_char\": 0, \"end_char\": 1863, \"text_sha256\": \"2ebbc851e5279e646bc7c892645c931a3fd2993830c3f4405379839cbbd6e707\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human erythrocytes, one G6PD-deficient donor and purified-enzyme assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lipoic acid with glucose/2-deoxyglucose and G6PD inhibition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The deficient-donor finding is small and mechanistic; it is not a clinical safety or efficacy trial in G6PD deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Providing the oxidized molecule did not overcome the missing reducing capacity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ala-p7632170] Reduction and transport of lipoic acid by human erythrocytes. 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