{"id":"97570e4a-c65b-529b-9ec4-778a0496214d","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-s6k-binding","predicate":"binds_and_inhibits","statement":"Cell-free competition and activity assays supported fisetin binding and inhibition of p70S6K in the human melanoma study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"fa1f9216-bef8-5b4b-9a27-34dc066e8973","mechanism_event_label":"A binding assay supports a more direct target relationship.","subject":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"object":{"id":"d357f815-7928-5fb8-a704-b526b6d6aa28","slug":"rps6kb1","display_name":"Human p70 S6 kinase / RPS6KB1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"fa1f9216-bef8-5b4b-9a27-34dc066e8973","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-s6k-binding-event","event_type":"observed_relationship","label":"A binding assay supports a more direct target relationship.","description":"Cell-free competition and activity assays supported fisetin binding and inhibition of p70S6K in the human melanoma study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d357f815-7928-5fb8-a704-b526b6d6aa28","slug":"rps6kb1","display_name":"Human p70 S6 kinase / RPS6KB1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human melanoma models plus cell-free kinase assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Docking was supplemented by biochemical assays; clinical target engagement remains unproven.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A binding assay supports a more direct target relationship.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Fisetin inhibits human melanoma cell growth through direct binding to p70S6K and mTOR: findings from 3-D melanoma skin equivalents and computational modeling. · 2014 · https://pubmed.ncbi.nlm.nih.gov/24675012/ · DOI 10.1016/j.bcp.2014.03.007","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9fdaf9e7-abe6-5b0b-a842-d840d2fa9893","evidence_kind":"source_excerpt","locator":"Lines 280-286","start_line":280,"end_line":286,"excerpt":"## fisetin-s6k-binding\nA binding assay supports a more direct target relationship.\nCell-free competition and activity assays supported fisetin binding and inhibition of p70S6K in the human melanoma study.\nModel: Human melanoma models plus cell-free kinase assays.\nLimitations: Docking was supplemented by biochemical assays; clinical target engagement remains unproven.\nEvidence access: Primary abstract\nFisetin inhibits human melanoma cell growth through direct binding to p70S6K and mTOR: findings from 3-D melanoma skin equivalents and computational modeling. · 2014 · https://pubmed.ncbi.nlm.nih.gov/24675012/ · DOI 10.1016/j.bcp.2014.03.007","model_system":"Human melanoma models plus cell-free kinase assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c30e0764cd34bd062cfcbfaf32357ef29dfda30e7d4153c545178140f5054e09","revision_id":"40ff52c0-f4b9-5820-8abb-ff002c6364a4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}