{"id":"9739063a-2ca9-5fe6-ba23-7fca0fce0164","stable_key":"research:apt2-depalmitoylates-zdhhc6","predicate":"depalmitoylates","statement":"APT2/LYPLA2 removes regulatory palmitoylation from ZDHHC6, with rapid turnover involving C328.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"neutral","is_public":true,"mechanism_event_id":"27a92487-ed13-53b9-96bf-eb48c53aad53","mechanism_event_label":"APT2 can remove ZDHHC6's regulatory lipid groups.","subject":{"id":"f2ffef5b-5457-50a8-b787-b8f24430f610","slug":"lypla2","display_name":"LYPLA2","entity_type_key":"protein"},"object":{"id":"05e9ea97-39b9-5e0a-8dde-6c337bdd67dd","slug":"regulatory-palmitoylated-zdhhc6","display_name":"Regulatory palmitoylated ZDHHC6","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"27a92487-ed13-53b9-96bf-eb48c53aad53","stable_key":"research:apt2-depalmitoylates-zdhhc6","event_type":"protein-deacylation","label":"APT2 can remove ZDHHC6's regulatory lipid groups.","description":"APT2/LYPLA2 removes regulatory palmitoylation from ZDHHC6, with rapid turnover involving C328.","status":"active","compartment":{"slug":"endoplasmic-reticulum","display_name":"Endoplasmic reticulum"},"participants":[{"entity":{"id":"f2ffef5b-5457-50a8-b787-b8f24430f610","slug":"lypla2","display_name":"LYPLA2","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"05e9ea97-39b9-5e0a-8dde-6c337bdd67dd","slug":"regulatory-palmitoylated-zdhhc6","display_name":"Regulatory palmitoylated ZDHHC6","entity_type_key":"protein_state"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a2fd4b35-5483-5e7c-9604-d792fa67d402","slug":"zdhhc6","display_name":"ZDHHC6","entity_type_key":"protein"},"role":"product","stoichiometry":null,"state_label":"depalmitoylated at regulatory sites","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"HeLa","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Enzyme perturbation and pulse-chase","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not removal of the catalytic acyl intermediate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"human","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1ce1ba05-44a0-536b-aadc-286273906418","evidence_kind":"curated_literature_summary","locator":"lines 894-904","start_line":894,"end_line":904,"excerpt":"## apt2-depalmitoylates-zdhhc6\n\nAPT2 can remove ZDHHC6's regulatory lipid groups.\n\nAPT2/LYPLA2 removes regulatory palmitoylation from ZDHHC6, with rapid turnover involving C328.\n\nOrganism: human\nCell type: HeLa\nExperimental model: Enzyme perturbation and pulse-chase\nLimitations: Not removal of the catalytic acyl intermediate.\nPrimary reference: [Identification and dynamics of the human ZDHHC16-ZDHHC6 palmitoylation cascade](https://elifesciences.org/articles/27826)","model_system":"Enzyme perturbation and pulse-chase","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"palmitoylation-regulation","plain_language":"APT2 can remove ZDHHC6's regulatory lipid groups.","evidence_scope":"direct_experimental","papers":[{"key":"abrami-2017-zdhhc-cascade","title":"Identification and dynamics of the human ZDHHC16-ZDHHC6 palmitoylation cascade","url":"https://elifesciences.org/articles/27826","doi":"10.7554/eLife.27826","year":2017,"model":"Human HeLa/HAP1 perturbations, cysteine mutants and kinetic modeling","summary":"Separates ZDHHC16-mediated regulatory palmitoylation from APT2-dependent removal and measures consequences for ZDHHC6 activity."}]}}