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The cells had ECHDC1 deleted.","subject":{"id":"b6d9b937-895d-5e0a-8cc5-0454a0b7d373","slug":"vitamin-b12","display_name":"Vitamin B12 (cobalamins)","entity_type_key":"chemical_species"},"object":{"id":"69ac0570-94d8-59d8-85ef-7f67cf302d16","slug":"propionyl-coa","display_name":"Propionyl-CoA","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"06dfd901-183f-5947-a518-73b2c884391f","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:l929-b12-lowers-propionyl-pool-event","event_type":"biochemical_relationship","label":"In the same mouse-cell experiment, B12 also lowered the upstream propionyl-CoA pool. The cells had ECHDC1 deleted.","description":"B12 supplementation lowered the measured propionyl-CoA pool approximately fourfold in differentiating ECHDC1-knockout L929 adipocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b6d9b937-895d-5e0a-8cc5-0454a0b7d373","slug":"vitamin-b12","display_name":"Vitamin B12 (cobalamins)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"69ac0570-94d8-59d8-85ef-7f67cf302d16","slug":"propionyl-coa","display_name":"Propionyl-CoA","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full text Results: vitamin B12 supplementation; Figure 4g-u","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Differentiating ECHDC1-knockout mouse L929 adipocytes (Figure 4g-u)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"ECHDC1 knockout; 2.5 micromolar supplementary B12 during differentiation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Whole-cell LC-MS pools; no compartment or epimer resolution and no direct pathway flux measurement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b12","display_name":"Vitamin B12 (cobalamins)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"In the same mouse-cell experiment, B12 also lowered the upstream propionyl-CoA pool. The cells had ECHDC1 deleted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[dewulf-2019-l929-b12] The synthesis of branched-chain fatty acids is limited by enzymatic decarboxylation of ethyl- and methylmalonyl-CoA. (2019). https://pubmed.ncbi.nlm.nih.gov/31416829/ DOI: 10.1042/bcj20190500","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Adipocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"5da5cc16-3a9a-51a1-bc88-a01eed6f16c7","evidence_kind":"source_excerpt","locator":"Lines 1272-1284","start_line":1272,"end_line":1284,"excerpt":"### l929-b12-lowers-propionyl-pool\nB12 supplementation lowered the measured propionyl-CoA pool approximately fourfold in differentiating ECHDC1-knockout L929 adipocytes.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: In the same mouse-cell experiment, B12 also lowered the upstream propionyl-CoA pool. The cells had ECHDC1 deleted.\norganism: Mus musculus\ntissue_or_cell_type: Adipocytes\nexperimental_model: Differentiating ECHDC1-knockout mouse L929 adipocytes (Figure 4g-u)\nlimitations: Whole-cell LC-MS pools; no compartment or epimer resolution and no direct pathway flux measurement.\nexposure: ECHDC1 knockout; 2.5 micromolar supplementary B12 during differentiation\ncross_nutrient: false\nevidence_location: Full text Results: vitamin B12 supplementation; Figure 4g-u\n[dewulf-2019-l929-b12] The synthesis of branched-chain fatty acids is limited by enzymatic decarboxylation of ethyl- and methylmalonyl-CoA. (2019). https://pubmed.ncbi.nlm.nih.gov/31416829/ DOI: 10.1042/bcj20190500","model_system":"Differentiating ECHDC1-knockout mouse L929 adipocytes (Figure 4g-u)","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [dewulf-2019-l929-b12] The synthesis of branched-chain fatty acids is limited by enzymatic decarboxylation of ethyl- and methylmalonyl-CoA. (2019). https://pubmed.ncbi.nlm.nih.gov/31416829/ DOI: 10.1042/bcj20190500","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73145023-2982-5848-aff2-8d8875d6b9c5","stable_key":"import-7fc92b9e-9cbf-556e-8719-6b5244625250","title":"Vitamin B12: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ad5b3a51d36e856aa7fdfd1cc23f690b094689bad87d28e1a621a54675a01c92","revision_id":"118bd616-0c13-549f-bfeb-bc439715b89c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}