{"id":"97168cf7-94fb-527c-bc14-d755890b7a60","stable_key":"d17caac5-7018-5ca8-9e96-95afeb384bc4:pectin-gal3-inhibition-limited","predicate":"shows_little_or_no_inhibition_of","statement":"Tested pectic preparations were weak or inactive canonical-site inhibitors; reported pectic IC50 values, where measurable, exceeded 10 mg/mL.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e8097488-6622-5dc8-ae0a-235ccce3762f","mechanism_event_label":"Attaching to a protein does not necessarily block its usual sugar-binding function.","subject":{"id":"cd34197d-e78f-5adb-80a9-eb423fb115c4","slug":"modified-citrus-pectin","display_name":"Modified citrus pectin, product-specific","entity_type_key":"chemical_species"},"object":{"id":"2289dc7b-77f6-54aa-b3ad-3384ce56df63","slug":"human-gal3-canonical-binding","display_name":"Human galectin-3 canonical carbohydrate-site binding","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e8097488-6622-5dc8-ae0a-235ccce3762f","stable_key":"d17caac5-7018-5ca8-9e96-95afeb384bc4:pectin-gal3-inhibition-limited-event","event_type":"biochemical_relationship","label":"Attaching to a protein does not necessarily block its usual sugar-binding function.","description":"Tested pectic preparations were weak or inactive canonical-site inhibitors; reported pectic IC50 values, where measurable, exceeded 10 mg/mL.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cbecd47d-620e-5afb-9e87-ae63442ed8ed","slug":"lgals3","display_name":"Human galectin-3 / LGALS3","entity_type_key":"protein"},"role":"tested_protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cd34197d-e78f-5adb-80a9-eb423fb115c4","slug":"modified-citrus-pectin","display_name":"Modified citrus pectin, product-specific","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2289dc7b-77f6-54aa-b3ad-3384ce56df63","slug":"human-gal3-canonical-binding","display_name":"Human galectin-3 canonical carbohydrate-site binding","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/pectin-research/27129206.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b9faed8d7344a109bfebf8e340281cc15375deb88a653cd129967a7084e9eb12\", \"start_char\": 0, \"end_char\": 1776, \"text_sha256\": \"b9faed8d7344a109bfebf8e340281cc15375deb88a653cd129967a7084e9eb12\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Fluorescence-anisotropy competition and cell assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Panel of bioactive pectins including modified products; synthetic pectic fragments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Preparation- and assay-specific. This challenges a general canonical-site blockade explanation; it does not disprove all physical interactions or every proposed noncanonical mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pectin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pectin","display_name":"Pectin, structurally heterogeneous plant polysaccharides","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Recombinant galectins and cultured cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Attaching to a protein does not necessarily block its usual sugar-binding function.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[pectin-p27129206] Low or No Inhibitory Potency of the Canonical Galectin Carbohydrate-binding Site by Pectins and Galactomannans. (2016). https://pubmed.ncbi.nlm.nih.gov/27129206/ DOI: 10.1074/jbc.m116.721464","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Canonical carbohydrate-binding site","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2bbdfcfe-d64b-53be-be45-9b1848604ed0","evidence_kind":"source_excerpt","locator":"Lines 464-475","start_line":464,"end_line":475,"excerpt":"### pectin-gal3-inhibition-limited\nTested pectic preparations were weak or inactive canonical-site inhibitors; reported pectic IC50 values, where measurable, exceeded 10 mg/mL.\nCondition category: normal\nnutrient_topic: Pectin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Attaching to a protein does not necessarily block its usual sugar-binding function.\norganism: Recombinant galectins and cultured cells\ntissue_or_cell_type: Canonical carbohydrate-binding site\nexperimental_model: Fluorescence-anisotropy competition and cell assays\nlimitations: Preparation- and assay-specific. This challenges a general canonical-site blockade explanation; it does not disprove all physical interactions or every proposed noncanonical mechanism.\nexposure: Panel of bioactive pectins including modified products; synthetic pectic fragments\nevidence_span: {\"source_cache\": \"artifacts/pectin-research/27129206.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b9faed8d7344a109bfebf8e340281cc15375deb88a653cd129967a7084e9eb12\", \"start_char\": 0, \"end_char\": 1776, \"text_sha256\": \"b9faed8d7344a109bfebf8e340281cc15375deb88a653cd129967a7084e9eb12\"}\n[pectin-p27129206] Low or No Inhibitory Potency of the Canonical Galectin Carbohydrate-binding Site by Pectins and Galactomannans. 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