{"id":"970a9907-5c69-5752-b86e-0fda14605f99","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-intestinal-resveratrol","predicate":"reduces","statement":"Resveratrol at 300 micromolar for 30 minutes reduced alanine-evoked short-circuit current in isolated porcine intestinal preparations.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"e2694a16-978c-5a42-8321-b7791f0e5ee0","mechanism_event_label":"A second compound altered the electrical transport response to alanine.","subject":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"object":{"id":"f3042028-febf-5014-97cf-7f5aa3396a70","slug":"pig-intestinal-alanine-current","display_name":"Alanine-evoked intestinal short-circuit current in pigs","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e2694a16-978c-5a42-8321-b7791f0e5ee0","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-intestinal-resveratrol-event","event_type":"observed_relationship","label":"A second compound altered the electrical transport response to alanine.","description":"Resveratrol at 300 micromolar for 30 minutes reduced alanine-evoked short-circuit current in isolated porcine intestinal preparations.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f3042028-febf-5014-97cf-7f5aa3396a70","slug":"pig-intestinal-alanine-current","display_name":"Alanine-evoked intestinal short-circuit current in pigs","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Pig jejunum/ileum Ussing chambers; alanine challenge.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Current is a functional proxy; associated AMPK/PKA changes were not established as the cause. No human malabsorption conclusion follows.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A second compound altered the electrical transport response to alanine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Resveratrol Inhibits Porcine Intestinal Glucose and Alanine Transport: Potential Roles of Na⁺/K⁺-ATPase Activity, Protein Kinase A, AMP-Activated Protein Kinase and the Association of Selected Nutrient Transport Proteins with Detergent Resistant Membranes. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29510506/ · DOI 10.3390/nu10030302","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2b390643-11a4-5e3b-b57a-d3623504b95f","evidence_kind":"source_excerpt","locator":"Lines 488-494","start_line":488,"end_line":494,"excerpt":"## alanine-intestinal-resveratrol\nA second compound altered the electrical transport response to alanine.\nResveratrol at 300 micromolar for 30 minutes reduced alanine-evoked short-circuit current in isolated porcine intestinal preparations.\nModel: Pig jejunum/ileum Ussing chambers; alanine challenge.\nLimitations: Current is a functional proxy; associated AMPK/PKA changes were not established as the cause. No human malabsorption conclusion follows.\nEvidence access: Primary full text\nResveratrol Inhibits Porcine Intestinal Glucose and Alanine Transport: Potential Roles of Na⁺/K⁺-ATPase Activity, Protein Kinase A, AMP-Activated Protein Kinase and the Association of Selected Nutrient Transport Proteins with Detergent Resistant Membranes. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29510506/ · DOI 10.3390/nu10030302","model_system":"Pig jejunum/ileum Ussing chambers; alanine challenge.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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