{"id":"96cc01ba-5cd4-5d19-b15a-395ecdea8c14","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-ampb-loss","predicate":"decreases_in_recorded_comparison","statement":"Deleting bacterial ampB eliminated the reported apigenin signal without the same loss of growth or colonization in the tested P. goldsteinii model.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"ddcac17e-1198-5486-ab86-07b2b4812164","mechanism_event_label":"Deleting bacterial ampB eliminated the reported apigenin signal without the same loss of growth or colonization in the tested P. goldsteinii model.","subject":{"id":"6c768fee-32d4-5ab8-bb33-8f5ed9c6cdaf","slug":"pgoldsteinii-ampb","display_name":"Parabacteroides goldsteinii AmpB candidate biosynthetic protein","entity_type_key":"protein"},"object":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"ddcac17e-1198-5486-ab86-07b2b4812164","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-ampb-loss-event","event_type":"experimental_observation","label":"Deleting bacterial ampB eliminated the reported apigenin signal without the same loss of growth or colonization in the tested P. goldsteinii model.","description":"### api-ampb-loss\n\nDeleting bacterial ampB eliminated the reported apigenin signal without the same loss of growth or colonization in the tested P. goldsteinii model.\nSupports a genetic requirement in this model; docking and structural similarity do not by themselves prove AmpB is a purified chalcone synthase.\nModel and exposure: Bacterial knockout and mouse colonization experiments.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Aging-induced Alternation in the Gut Microbiota Impairs Host Antibacterial Defense. | 2025 | https://pubmed.ncbi.nlm.nih.gov/39792643/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11948050/ | https://doi.org/10.1002/advs.202411008 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6c768fee-32d4-5ab8-bb33-8f5ed9c6cdaf","slug":"pgoldsteinii-ampb","display_name":"Parabacteroides goldsteinii AmpB candidate biosynthetic protein","entity_type_key":"protein"},"role":"tested factor","stoichiometry":null,"state_label":"ampB knockout","sequence_order":0,"notes":""},{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"528bf013-ff85-5bdb-8551-5b83d990c741","slug":"pgoldsteinii","display_name":"Parabacteroides goldsteinii","entity_type_key":"organism"},"role":"experimental organism","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"ampB knockout\", \"comparator\": \"Wild-type bacterium\", \"endpoint\": \"Deleting bacterial ampB eliminated the reported apigenin signal without the same loss of growth or colonization in the tested P. goldsteinii model.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Bacterial knockout and mouse colonization experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Deleting bacterial ampB eliminated the reported apigenin signal without the same loss of growth or colonization in the tested P. goldsteinii model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Aging-induced Alternation in the Gut Microbiota Impairs Host Antibacterial Defense. | https://pubmed.ncbi.nlm.nih.gov/39792643/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11948050/ | https://doi.org/10.1002/advs.202411008","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-ampb-loss; Supports a genetic requirement in this model; docking and structural similarity do not by themselves prove AmpB is a purified chalcone synthase.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"2924bdf1-1019-5b07-9af4-c9c0aba04942","evidence_kind":"source_excerpt","locator":"Lines 480-486","start_line":480,"end_line":486,"excerpt":"### api-ampb-loss\n\nDeleting bacterial ampB eliminated the reported apigenin signal without the same loss of growth or colonization in the tested P. goldsteinii model.\nSupports a genetic requirement in this model; docking and structural similarity do not by themselves prove AmpB is a purified chalcone synthase.\nModel and exposure: Bacterial knockout and mouse colonization experiments.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Aging-induced Alternation in the Gut Microbiota Impairs Host Antibacterial Defense. | 2025 | https://pubmed.ncbi.nlm.nih.gov/39792643/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11948050/ | https://doi.org/10.1002/advs.202411008 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"Bacterial knockout and mouse colonization experiments.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}