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unverified.","entity":null}],"evidence":[{"id":"60402ab6-ff28-5e26-9a63-d0252038abc6","evidence_kind":"source_excerpt","locator":"Lines 555-564","start_line":555,"end_line":564,"excerpt":"### folate-methyl-cblg-methyl-trap\nThe fraction of measured intracellular folate present as 5-methyl-THF was 2.5-fold higher in cblG WG4215 fibroblasts than in control MCH058 fibroblasts.\nCondition category: machinery_impairment\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Genetic MTR loss shifted the measured folate mixture toward methylfolate.\norganism: Homo sapiens\ntissue_or_cell_type: Patient fibroblasts\nexperimental_model: N2O-treated HeLa cells and cblG patient fibroblasts.\nlimitations: Fig. 2A reports folate-species percentages, not absolute amounts; one disease/control cell-line comparison.\n[palmer-2017] Folate rescues vitamin B12 depletion-induced inhibition of nuclear thymidylate biosynthesis and genome instability (2017). https://pubmed.ncbi.nlm.nih.gov/28461497/ DOI: 10.1073/pnas.1619582114","model_system":"N2O-treated HeLa cells and cblG patient fibroblasts.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [palmer-2017] Folate rescues vitamin B12 depletion-induced inhibition of nuclear thymidylate biosynthesis and genome instability (2017). https://pubmed.ncbi.nlm.nih.gov/28461497/ DOI: 10.1073/pnas.1619582114","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}