{"id":"96b4b0ab-e889-50f0-b5a4-cc2a069eb27e","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-sat1-substrate","predicate":"acetylates","statement":"Human SAT1 catalyzes N1-acetylation of spermidine using acetyl-CoA.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8ec2966b-c9cc-5f75-9653-57e9a7f96a31","mechanism_event_label":"A CoA-dependent step changes how spermidine is recycled.","subject":{"id":"3763e5e6-9a10-5a6a-a39c-8c53fc190002","slug":"sat1","display_name":"Human spermidine/spermine N1-acetyltransferase / SAT1","entity_type_key":"protein"},"object":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"8ec2966b-c9cc-5f75-9653-57e9a7f96a31","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-sat1-substrate-event","event_type":"observed_relationship","label":"A CoA-dependent step changes how spermidine is recycled.","description":"Human SAT1 catalyzes N1-acetylation of spermidine using acetyl-CoA.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3763e5e6-9a10-5a6a-a39c-8c53fc190002","slug":"sat1","display_name":"Human spermidine/spermine N1-acetyltransferase / SAT1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"69481d06-17fc-50ee-87f6-81c958322f65","slug":"n1-acetylspermidine","display_name":"N1-Acetylspermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified recombinant human SAT1 kinetics and structural analysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Acetyl-CoA dependence connects to CoA metabolism, not demonstrated pantothenate depletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A CoA-dependent step changes how spermidine is recycled.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mechanistic and structural analysis of human spermidine/spermine N1-acetyltransferase. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17516632/ · DOI 10.1021/bi700256z","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a9f6967d-0084-5944-bed3-10fcdc80b4ac","evidence_kind":"source_excerpt","locator":"Lines 78-84","start_line":78,"end_line":84,"excerpt":"## spermidine-sat1-substrate\nA CoA-dependent step changes how spermidine is recycled.\nHuman SAT1 catalyzes N1-acetylation of spermidine using acetyl-CoA.\nModel: Purified recombinant human SAT1 kinetics and structural analysis.\nLimitations: Acetyl-CoA dependence connects to CoA metabolism, not demonstrated pantothenate depletion.\nEvidence access: Primary abstract\nMechanistic and structural analysis of human spermidine/spermine N1-acetyltransferase. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17516632/ · DOI 10.1021/bi700256z","model_system":"Purified recombinant human SAT1 kinetics and structural analysis.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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