{"id":"96a8b059-acaa-5871-b158-5b27c24787f5","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-cd36-knockout","predicate":"reduced","statement":"Adipose explants from Cd36-null mice took up less lycopene than wild-type explants.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"754dc441-41b1-5ebf-a040-d8b500fff9b1","mechanism_event_label":"Removing this transporter reduced entry into mouse fat tissue.","subject":{"id":"849efcdc-02b0-5aec-9675-f243b4432279","slug":"mouse-cd36-knockout","display_name":"Mouse Cd36 knockout","entity_type_key":"protein_state"},"object":{"id":"aab7ad04-1a7b-5385-810c-ec5ac123af74","slug":"mouse-adipose-lycopene-uptake","display_name":"Lycopene uptake by mouse adipose explants","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"754dc441-41b1-5ebf-a040-d8b500fff9b1","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-cd36-knockout-event","event_type":"biochemical_relationship","label":"Removing this transporter reduced entry into mouse fat tissue.","description":"Adipose explants from Cd36-null mice took up less lycopene than wild-type explants.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0b16e027-7a68-55ae-8e33-eae8b5cd1e9e","slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"},"role":"transported_carotenoid","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"849efcdc-02b0-5aec-9675-f243b4432279","slug":"mouse-cd36-knockout","display_name":"Mouse Cd36 knockout","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"aab7ad04-1a7b-5385-810c-ec5ac123af74","slug":"mouse-adipose-lycopene-uptake","display_name":"Lycopene uptake by mouse adipose explants","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/lycopene-research/21462325.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"de4990e5a9c520e6e528e181a4e81a469a4ebac313151bfa7828ad935b04a823\", \"start_char\": 0, \"end_char\": 1048, \"text_sha256\": \"de4990e5a9c520e6e528e181a4e81a469a4ebac313151bfa7828ad935b04a823\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Transport inhibition, siRNA, overexpression and knockout explants","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"CD36 inhibitor, partial knockdown and Cd36-null tissue","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not relabel adipose results as intestinal uptake or a clinical human transport-deficiency syndrome.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lycopene research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mouse 3T3-L1 adipocytes and mouse adipose explants; COS-1 expression experiment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Removing this transporter reduced entry into mouse fat tissue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lycopene-p21462325] CD36 is involved in lycopene and lutein uptake by adipocytes and adipose tissue cultures. (2011). https://pubmed.ncbi.nlm.nih.gov/21462325/ DOI: 10.1002/mnfr.201000399","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Adipocytes and adipose tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3b36bdfe-7e42-5b70-a02b-cb3ffb351db9","evidence_kind":"source_excerpt","locator":"Lines 273-284","start_line":273,"end_line":284,"excerpt":"### lycopene-cd36-knockout\nAdipose explants from Cd36-null mice took up less lycopene than wild-type explants.\nCondition category: normal\nnutrient_topic: Lycopene research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Removing this transporter reduced entry into mouse fat tissue.\norganism: Mouse 3T3-L1 adipocytes and mouse adipose explants; COS-1 expression experiment\ntissue_or_cell_type: Adipocytes and adipose tissue\nexperimental_model: Transport inhibition, siRNA, overexpression and knockout explants\nlimitations: Do not relabel adipose results as intestinal uptake or a clinical human transport-deficiency syndrome.\nexposure: CD36 inhibitor, partial knockdown and Cd36-null tissue\nevidence_span: {\"source_cache\": \"artifacts/lycopene-research/21462325.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"de4990e5a9c520e6e528e181a4e81a469a4ebac313151bfa7828ad935b04a823\", \"start_char\": 0, \"end_char\": 1048, \"text_sha256\": \"de4990e5a9c520e6e528e181a4e81a469a4ebac313151bfa7828ad935b04a823\"}\n[lycopene-p21462325] CD36 is involved in lycopene and lutein uptake by adipocytes and adipose tissue cultures. 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