{"id":"9658d905-be4c-52b6-9e53-aa91b3e979f7","stable_key":"fe6af7fc-7372-5c4f-9c2f-2bbf56695397:phosphorus-rbc-lactate","predicate":"increases","statement":"Raising incubation Pi from 1 to 5 mmol/L increased normal-donor erythrocyte lactate production from 2.09 to 3.11 mmol/hour/L cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f7be6a09-308f-5d2f-9bc2-db42cfe01d50","mechanism_event_label":"More phosphate accelerated the measured glycolytic output in this cell preparation.","subject":{"id":"ee63fe8e-92f3-552f-8049-a5f1fa1e1523","slug":"phosphate-ion","display_name":"Inorganic phosphate (Pi; protonation depends on pH)","entity_type_key":"ion"},"object":{"id":"d4fab92c-f7d3-53ff-bcf7-0c3938bc34b6","slug":"erythrocyte-lactate-production","display_name":"Human erythrocyte lactate production rate","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f7be6a09-308f-5d2f-9bc2-db42cfe01d50","stable_key":"fe6af7fc-7372-5c4f-9c2f-2bbf56695397:phosphorus-rbc-lactate-event","event_type":"biochemical_relationship","label":"More phosphate accelerated the measured glycolytic output in this cell preparation.","description":"Raising incubation Pi from 1 to 5 mmol/L increased normal-donor erythrocyte lactate production from 2.09 to 3.11 mmol/hour/L cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"glycolytic_context","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ee63fe8e-92f3-552f-8049-a5f1fa1e1523","slug":"phosphate-ion","display_name":"Inorganic phosphate (Pi; protonation depends on pH)","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d4fab92c-f7d3-53ff-bcf7-0c3938bc34b6","slug":"erythrocyte-lactate-production","display_name":"Human erythrocyte lactate production rate","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/phosphorus-research/4042545.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"474d2b2722565fa0e4c2f0ddf4a332715ce176f9325b9af5c22027bd6ac93e43\", \"start_char\": 0, \"end_char\": 1711, \"text_sha256\": \"474d2b2722565fa0e4c2f0ddf4a332715ce176f9325b9af5c22027bd6ac93e43\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"ICU erythrocyte measurements and normal-donor cell incubation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Plasma Pi 0.1–4.2 mmol/L in patients; normal-donor cells incubated at 1 or 5 mmol/L Pi","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Observed ranges are not universal thresholds; ATP concentration, ATP synthetic flux and 2,3-DPG are separate endpoints.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Phosphorus research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"phosphorus","display_name":"Phosphorus","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"More phosphate accelerated the measured glycolytic output in this cell preparation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[phosphorus-p4042545] Phosphate metabolism in erythrocytes of critically ill patients. (1985). https://pubmed.ncbi.nlm.nih.gov/4042545/ DOI: 10.1042/cs0690435","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Red blood cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"420ed70f-5e6e-50d7-bd7a-cb675c709e7a","evidence_kind":"source_excerpt","locator":"Lines 1089-1100","start_line":1089,"end_line":1100,"excerpt":"### phosphorus-rbc-lactate\nRaising incubation Pi from 1 to 5 mmol/L increased normal-donor erythrocyte lactate production from 2.09 to 3.11 mmol/hour/L cells.\nCondition category: normal\nnutrient_topic: Phosphorus research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: More phosphate accelerated the measured glycolytic output in this cell preparation.\norganism: Human\ntissue_or_cell_type: Red blood cells\nexperimental_model: ICU erythrocyte measurements and normal-donor cell incubation\nlimitations: Observed ranges are not universal thresholds; ATP concentration, ATP synthetic flux and 2,3-DPG are separate endpoints.\nexposure: Plasma Pi 0.1–4.2 mmol/L in patients; normal-donor cells incubated at 1 or 5 mmol/L Pi\nevidence_span: {\"source_cache\": \"artifacts/phosphorus-research/4042545.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"474d2b2722565fa0e4c2f0ddf4a332715ce176f9325b9af5c22027bd6ac93e43\", \"start_char\": 0, \"end_char\": 1711, \"text_sha256\": \"474d2b2722565fa0e4c2f0ddf4a332715ce176f9325b9af5c22027bd6ac93e43\"}\n[phosphorus-p4042545] Phosphate metabolism in erythrocytes of critically ill patients. 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