{"id":"95a38a96-b8e2-5c42-8ec7-98df38656cbd","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-tfeb-biogenesis","predicate":"supports","statement":"Autophagy and lysosomal biogenesis through TFEB required MCOLN1-mediated calcineurin activation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"cddb1ad8-2f23-5f16-b03a-cff0b2d1121a","mechanism_event_label":"The signal helped build the cell’s recycling capacity.","subject":{"id":"a04514ba-11bb-5809-8d2f-75ca964fad15","slug":"tfeb","display_name":"Human transcription factor EB / TFEB","entity_type_key":"protein"},"object":{"id":"31c0ea4d-9924-5a03-9bb1-fdd3013c34b1","slug":"lysosomal-biogenesis","display_name":"Lysosomal biogenesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"cddb1ad8-2f23-5f16-b03a-cff0b2d1121a","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-tfeb-biogenesis-event","event_type":"observed_relationship","label":"The signal helped build the cell’s recycling capacity.","description":"Autophagy and lysosomal biogenesis through TFEB required MCOLN1-mediated calcineurin activation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a04514ba-11bb-5809-8d2f-75ca964fad15","slug":"tfeb","display_name":"Human transcription factor EB / TFEB","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"31c0ea4d-9924-5a03-9bb1-fdd3013c34b1","slug":"lysosomal-biogenesis","display_name":"Lysosomal biogenesis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6d278f80-c1e5-53b9-9c4d-03e1ed31de2a","slug":"mcoln1","display_name":"Human mucolipin-1 / MCOLN1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"27eef74a-1d9b-57b3-a245-82d452d6cb54","slug":"calcineurin","display_name":"Calcineurin","entity_type_key":"protein_complex"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"8f3e8b4e-812d-5a5c-8b34-ebfd4cfd0d26","slug":"cellular-autophagic-flux","display_name":"Cellular autophagic flux, experimental context specified","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cellular starvation and mouse exercise experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Lysosome abundance alone is not proof of increased degradation flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fasting physiological-state collection; human protocols, cellular deprivation and refeeding are distinguished.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fasting","display_name":"Fasting / abstention from energy intake","entity_type_key":"cellular_process"}},{"dimension":"plain_language","value_text":"The signal helped build the cell’s recycling capacity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Lysosomal calcium signalling regulates autophagy through calcineurin and ​TFEB. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25720963/ · DOI 10.1038/ncb3114","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b687eb31-82ef-52c5-98b9-f40f7180023e","evidence_kind":"source_excerpt","locator":"Lines 344-350","start_line":344,"end_line":350,"excerpt":"## fast-tfeb-biogenesis\nThe signal helped build the cell’s recycling capacity.\nAutophagy and lysosomal biogenesis through TFEB required MCOLN1-mediated calcineurin activation.\nModel: Cellular starvation and mouse exercise experiments.\nLimitations: Lysosome abundance alone is not proof of increased degradation flux.\nEvidence access: Primary abstract\nLysosomal calcium signalling regulates autophagy through calcineurin and ​TFEB. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25720963/ · DOI 10.1038/ncb3114","model_system":"Cellular starvation and mouse exercise experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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