{"id":"959b7aca-a876-5712-b8dd-1868af873ec9","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-ninj1-necroptosis-limit","predicate":"does_not_protect_tested","statement":"Glycine did not protect primary human macrophages from the tested zVAD/BV6/TNF-induced necroptotic rupture.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"38226730-2f8d-5205-8af1-1ea433ffc131","mechanism_event_label":"The same protection did not apply to every route of cell death.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"8d23ce91-90d3-5909-a938-71a60c16727d","slug":"human-macrophage-necroptotic-rupture","display_name":"Necroptotic rupture in human macrophages","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"38226730-2f8d-5205-8af1-1ea433ffc131","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-ninj1-necroptosis-limit-event","event_type":"observed_relationship","label":"The same protection did not apply to every route of cell death.","description":"Glycine did not protect primary human macrophages from the tested zVAD/BV6/TNF-induced necroptotic rupture.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8d23ce91-90d3-5909-a938-71a60c16727d","slug":"human-macrophage-necroptotic-rupture","display_name":"Necroptotic rupture in human macrophages","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0784250d-c308-508d-8805-a0ed777463d5","slug":"ninj1","display_name":"Human ninjurin-1 / NINJ1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary human macrophages under a defined necroptosis protocol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Different death execution pathways explain a context boundary; do not label this a conflicting replication of pyroptosis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The same protection did not apply to every route of cell death.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Glycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36468682/ · DOI 10.7554/eLife.78609","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"41c6a703-48a3-53f7-8395-60d20c187efb","evidence_kind":"source_excerpt","locator":"Lines 378-384","start_line":378,"end_line":384,"excerpt":"## glycine-ninj1-necroptosis-limit\nThe same protection did not apply to every route of cell death.\nGlycine did not protect primary human macrophages from the tested zVAD/BV6/TNF-induced necroptotic rupture.\nModel: Primary human macrophages under a defined necroptosis protocol.\nLimitations: Different death execution pathways explain a context boundary; do not label this a conflicting replication of pyroptosis.\nEvidence access: Primary full text\nGlycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36468682/ · DOI 10.7554/eLife.78609","model_system":"Primary human macrophages under a defined necroptosis protocol.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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