{"id":"958802c8-fa1b-5ff0-949f-8ec5f06163d4","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:lysine-deprivation-mtorc1","predicate":"deprivation_suppresses_and_repletion_restores","statement":"Lysine withdrawal suppressed mTORC1 activity in NSCLC cell lines, and lysine restoration reversed the suppression; GCN2 and AMPK contributed to this response.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ab161a8e-872f-5b1c-8c59-1d8baca3803e","mechanism_event_label":"These cultured cancer cells needed available lysine for full nutrient-and-growth-factor signaling.","subject":{"id":"5fff6664-209e-5c35-b88e-ac15c1c5fbc0","slug":"l-lysine","display_name":"L-Lysine","entity_type_key":"small_molecule"},"object":{"id":"9f025af3-f039-517f-908b-4af6fc65415e","slug":"mtorc1","display_name":"Mechanistic target of rapamycin complex 1","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"ab161a8e-872f-5b1c-8c59-1d8baca3803e","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:lysine-deprivation-mtorc1-event","event_type":"biochemical_relationship","label":"These cultured cancer cells needed available lysine for full nutrient-and-growth-factor signaling.","description":"Lysine withdrawal suppressed mTORC1 activity in NSCLC cell lines, and lysine restoration reversed the suppression; 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unverified.","entity":null},{"dimension":"experimental_model","value_text":"Human NSCLC cell cultures including H1299, H460, and A549","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Abrupt medium depletion; no direct lysine-binding sensor established; no supplementation benefit inferred for healthy humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"These cultured cancer cells needed available lysine for full nutrient-and-growth-factor signaling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[jang2020] Lysine is required for growth factor-induced mTORC1 activation (2020). https://pubmed.ncbi.nlm.nih.gov/33008594/ DOI: 10.1016/j.bbrc.2020.09.100","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cultured lung cancer cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; 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Original study references: [jang2020] Lysine is required for growth factor-induced mTORC1 activation (2020). https://pubmed.ncbi.nlm.nih.gov/33008594/ DOI: 10.1016/j.bbrc.2020.09.100","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}