{"id":"95822f15-9bc3-5c2a-a5f9-32c69d7ab39b","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-glucuronide-gclc","predicate":"increases_expression","statement":"Luteolin 7-O-glucuronide increased GCLC expression alongside Nrf2 activation in RAW264.7 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"516f02bb-2ffa-582e-bfe5-e08ffc06ea96","mechanism_event_label":"A conjugate can influence glutathione-synthesis machinery.","subject":{"id":"4afd87a9-44dd-5774-8348-3dc09cde3e2a","slug":"luteolin-7-o-glucuronide","display_name":"Luteolin 7-O-glucuronide","entity_type_key":"small_molecule"},"object":{"id":"142ffffe-0359-5079-aba0-065f1fa198c4","slug":"mouse-gclc","display_name":"Mouse glutamate-cysteine ligase catalytic subunit / Gclc","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"516f02bb-2ffa-582e-bfe5-e08ffc06ea96","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-glucuronide-gclc-event","event_type":"observed_relationship","label":"A conjugate can influence glutathione-synthesis machinery.","description":"Luteolin 7-O-glucuronide increased GCLC expression alongside Nrf2 activation in RAW264.7 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4afd87a9-44dd-5774-8348-3dc09cde3e2a","slug":"luteolin-7-o-glucuronide","display_name":"Luteolin 7-O-glucuronide","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"142ffffe-0359-5079-aba0-065f1fa198c4","slug":"mouse-gclc","display_name":"Mouse glutamate-cysteine ligase catalytic subunit / Gclc","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"95319607-3946-51b3-8235-400ec3223692","slug":"mouse-nfe2l2","display_name":"Mouse Nrf2 / Nfe2l2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse macrophage culture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression alone does not show human glutathione restoration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A conjugate can influence glutathione-synthesis machinery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Anti-Inflammatory and Anti-Oxidative Effects of luteolin-7-O-glucuronide in LPS-Stimulated Murine Macrophages through TAK1 Inhibition and Nrf2 Activation. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32187984/ · DOI 10.3390/ijms21062007","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2a869a00-3219-5b8a-a90d-157df2e204d9","evidence_kind":"source_excerpt","locator":"Lines 508-514","start_line":508,"end_line":514,"excerpt":"## luteolin-glucuronide-gclc\nA conjugate can influence glutathione-synthesis machinery.\nLuteolin 7-O-glucuronide increased GCLC expression alongside Nrf2 activation in RAW264.7 cells.\nModel: Mouse macrophage culture.\nLimitations: Expression alone does not show human glutathione restoration.\nEvidence access: Primary full text\nAnti-Inflammatory and Anti-Oxidative Effects of luteolin-7-O-glucuronide in LPS-Stimulated Murine Macrophages through TAK1 Inhibition and Nrf2 Activation. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32187984/ · DOI 10.3390/ijms21062007","model_system":"Mouse macrophage culture.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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