{"id":"956d53c9-893e-564a-b341-ffde3cf9af46","stable_key":"16c35a72-8162-5bb1-ae01-45331b8b42d0:moringa-brusatol-blocks-nrf2-response","predicate":"diminishes","statement":"Brusatol treatment significantly diminished the ME-D-induced increase in NQO1 expression in BEAS-2B cells, supporting NRF2 dependence for that measured response.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"0e31275f-e617-5d35-a7a6-7e48810a3db2","mechanism_event_label":"Brusatol treatment significantly diminished the ME-D-induced increase in NQO1 expression in BEAS-2B cells, supporting NRF2 dependence for that measured response.","subject":{"id":"7af76172-7ab4-50f5-ab38-4c749e5533a1","slug":"brusatol","display_name":"Brusatol","entity_type_key":"drug"},"object":{"id":"0ce3909d-9c1b-5959-85e2-0e6a20fec4c8","slug":"moringa-nrf2-target-response","display_name":"NRF2-linked NQO1, HMOX1 and glutathione response to ME-D","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0e31275f-e617-5d35-a7a6-7e48810a3db2","stable_key":"16c35a72-8162-5bb1-ae01-45331b8b42d0:moringa-brusatol-blocks-nrf2-response-event","event_type":"observed_relationship","label":"Brusatol treatment significantly diminished the ME-D-induced increase in NQO1 expression in BEAS-2B cells, supporting NRF2 dependence for that measured response.","description":"Brusatol treatment significantly diminished the ME-D-induced increase in NQO1 expression in BEAS-2B cells, supporting NRF2 dependence for that measured response.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7af76172-7ab4-50f5-ab38-4c749e5533a1","slug":"brusatol","display_name":"Brusatol","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0ce3909d-9c1b-5959-85e2-0e6a20fec4c8","slug":"moringa-nrf2-target-response","display_name":"NRF2-linked NQO1, HMOX1 and glutathione response to ME-D","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fef56d1b-5db0-5bd8-8dd9-47539e31dce6","slug":"moringa-me-d-preparation","display_name":"Standardized palatable Moringa leaf preparation ME-D","entity_type_key":"botanical"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"dc472dd3-d383-567a-ab37-a9ec4e112df4","slug":"nfe2l2","display_name":"Human Nrf2 / NFE2L2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"01f73291-ab25-59e2-a5e9-71d15a6c2a0a","slug":"nqo1","display_name":"Human NAD(P)H quinone dehydrogenase 1 / NQO1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"dose","value_text":"ME-D with brusatol","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. 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(2023). https://pubmed.ncbi.nlm.nih.gov/37114361/ DOI: 10.1039/d3fo00572k\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Human BEAS-2B cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2752e9b3-e4b9-55fe-be07-944204cca4b8","stable_key":"import-16c35a72-8162-5bb1-ae01-45331b8b42d0","title":"Moringa oleifera: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. 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