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(2021). https://pubmed.ncbi.nlm.nih.gov/31409554/ DOI: 10.1016/j.phymed.2019.153062","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HDAC6, AKT and mTOR signaling","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ff1ff58c-fe56-5548-9589-3a49c4442abb","evidence_kind":"source_excerpt","locator":"Lines 632-643","start_line":632,"end_line":643,"excerpt":"### sulforaphane-mtor-independent\nSulforaphane inhibited mTOR signaling in U2OS cells both with and without NRF2.\nCondition category: normal\nnutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Some effects remain when the familiar NRF2 pathway is absent.\norganism: Human U2OS osteosarcoma cells and NRF2-knockout counterpart\ntissue_or_cell_type: HDAC6, AKT and mTOR signaling\nexperimental_model: Human cell experiments with CRISPR NRF2 deletion\nlimitations: Preclinical cancer-cell signaling; AKT phosphorylation did not reliably represent catalytic output. Not a human longevity or autophagy trial.\nexposure: Concentration- and time-dependent sulforaphane treatment\nevidence_span: {\"source_cache\": \"artifacts/sulforaphane-research/31409554.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5486f97c1271a771620cdf2e6245a54ff63f3f56b3eb884f201fda9882ec947b\", \"start_char\": 0, \"end_char\": 1778, \"text_sha256\": \"5486f97c1271a771620cdf2e6245a54ff63f3f56b3eb884f201fda9882ec947b\"}\n[sulforaphane-p31409554] The isothiocyanate sulforaphane inhibits mTOR in an NRF2-independent manner. (2021). https://pubmed.ncbi.nlm.nih.gov/31409554/ DOI: 10.1016/j.phymed.2019.153062","model_system":"Human cell experiments with CRISPR NRF2 deletion","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [sulforaphane-p31409554] The isothiocyanate sulforaphane inhibits mTOR in an NRF2-independent manner. (2021). https://pubmed.ncbi.nlm.nih.gov/31409554/ DOI: 10.1016/j.phymed.2019.153062","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"a4bafbf7-f117-53c9-a6d7-8fb25e4aca72","stable_key":"import-44eaeae5-557a-552b-8998-884f30462e2a","title":"Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f355c492a6c35673d8260b1328667fe206e5315b9fcaa5aeff53d80373c7ef97","revision_id":"f2c47d71-34cd-5160-8e92-fe196b576707","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}