{"id":"94ec739e-e7a3-5e9f-9233-c02853c5b477","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-peroxynitrite-nitration","predicate":"reduces","statement":"Ergothioneine protected against peroxynitrite-induced tyrosine nitration in vitro.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"ea48f78b-c543-5613-bb77-4e3cb2f204b1","mechanism_event_label":"Protection includes a nitrogen-derived oxidant.","subject":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"object":{"id":"3cd12cac-a909-59eb-9618-72b17bc2d0ca","slug":"chemical-tyrosine-nitration","display_name":"Tyrosine nitration in peroxynitrite assays","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ea48f78b-c543-5613-bb77-4e3cb2f204b1","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-peroxynitrite-nitration-event","event_type":"observed_relationship","label":"Protection includes a nitrogen-derived oxidant.","description":"Ergothioneine protected against peroxynitrite-induced tyrosine nitration in vitro.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3cd12cac-a909-59eb-9618-72b17bc2d0ca","slug":"chemical-tyrosine-nitration","display_name":"Tyrosine nitration in peroxynitrite assays","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Chemical antioxidant assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not evidence of removal of all reactive nitrogen species in vivo.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Protection includes a nitrogen-derived oxidant.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Antioxidant action of ergothioneine: assessment of its ability to scavenge peroxynitrite. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9070285/ · DOI 10.1006/bbrc.1997.6109","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"10fdd9d6-e937-526b-be4c-0d75560783b7","evidence_kind":"source_excerpt","locator":"Lines 152-158","start_line":152,"end_line":158,"excerpt":"## ergothioneine-peroxynitrite-nitration\nProtection includes a nitrogen-derived oxidant.\nErgothioneine protected against peroxynitrite-induced tyrosine nitration in vitro.\nModel: Chemical antioxidant assays.\nLimitations: Not evidence of removal of all reactive nitrogen species in vivo.\nEvidence access: Primary abstract\nAntioxidant action of ergothioneine: assessment of its ability to scavenge peroxynitrite. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9070285/ · DOI 10.1006/bbrc.1997.6109","model_system":"Chemical antioxidant assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b6def8119f03cfabeec2fb55ba924340dc612e1dd42f05404201fa2b7ced5259","revision_id":"f88c38e6-ffb5-5a68-83ca-4610ca2c2df4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}