{"id":"946697a3-89ce-522e-82b5-889e4f5a42b7","stable_key":"research:manganese-supports-selenoo-nadase","predicate":"supports","statement":"Mn2+ supports the reported SELENOO NAD-hydrolysis activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"positive","is_public":true,"mechanism_event_id":"2481febc-986b-5b85-8bbe-5710bb1e2ba8","mechanism_event_label":"Manganese acts as a cofactor in this reaction.","subject":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"object":{"id":"90e4a389-1062-5489-bae1-d49f337c98f0","slug":"selenoo","display_name":"SELENOO","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"2481febc-986b-5b85-8bbe-5710bb1e2ba8","stable_key":"research:manganese-supports-selenoo-nadase","event_type":"cofactor-dependence","label":"Manganese acts as a cofactor in this reaction.","description":"Mn2+ supports the reported SELENOO NAD-hydrolysis activity.","status":"active","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"90e4a389-1062-5489-bae1-d49f337c98f0","slug":"selenoo","display_name":"SELENOO","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"experimental cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Metal-dependent enzyme assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cofactor dependence does not establish nutritional limitation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"mammalian","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0910d3f5-ff2b-58bd-ac5a-53544352c98e","evidence_kind":"curated_literature_summary","locator":"lines 450-460","start_line":450,"end_line":460,"excerpt":"## manganese-supports-selenoo-nadase\n\nManganese acts as a cofactor in this reaction.\n\nMn2+ supports the reported SELENOO NAD-hydrolysis activity.\n\nOrganism: mammalian\nCell type: experimental cells\nExperimental model: Metal-dependent enzyme assays\nLimitations: Cofactor dependence does not establish nutritional limitation.\nPrimary reference: [NAD+ hydrolysis catalyzed by SelO is required for mitochondrial homeostasis](https://pubmed.ncbi.nlm.nih.gov/41806834/)","model_system":"Metal-dependent enzyme assays","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"mineral-cofactors","plain_language":"Manganese acts as a cofactor in this reaction.","evidence_scope":"direct_experimental","papers":[{"key":"jia-2026-selenoo-nad","title":"NAD+ hydrolysis catalyzed by SelO is required for mitochondrial homeostasis","url":"https://pubmed.ncbi.nlm.nih.gov/41806834/","doi":"10.1016/j.cell.2026.01.033","year":2026,"model":"Biochemistry and mammalian-cell experiments; additional bacterial work","summary":"Identifies manganese-dependent NAD cleavage by SELENOO and a requirement for its C-terminal Sec-containing region."}]}}