{"id":"94443632-a655-557c-83b1-22289717fd30","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-gsh-carrier","predicate":"reverses_inhibition","statement":"Adding reduced glutathione reversed copper inhibition of the reconstituted native rat carnitine-acylcarnitine carrier.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ea82ec93-6d14-5fa5-b57a-f00d405b887a","mechanism_event_label":"The redox environment can change whether the carrier works.","subject":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"object":{"id":"ee012f58-8529-5dab-a512-5236fb2b3d50","slug":"rat-cact-transport-activity","display_name":"Rat mitochondrial CACT transport activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ea82ec93-6d14-5fa5-b57a-f00d405b887a","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-gsh-carrier-event","event_type":"observed_relationship","label":"The redox environment can change whether the carrier works.","description":"Adding reduced glutathione reversed copper inhibition of the reconstituted native rat carnitine-acylcarnitine carrier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ee012f58-8529-5dab-a512-5236fb2b3d50","slug":"rat-cact-transport-activity","display_name":"Rat mitochondrial CACT transport activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"49164668-8ef7-5bde-afda-1c6aadd87c68","slug":"rat-slc25a20","display_name":"Rat carnitine-acylcarnitine translocase / Slc25a20","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2a870bb5-05a0-5e51-a70c-a9a843a8b571","slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat-liver CACT proteoliposome assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not demonstrate oral glutathione-carnitine synergy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Carnitine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The redox environment can change whether the carrier works.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Effect of Copper on the Mitochondrial Carnitine/Acylcarnitine Carrier Via Interaction with Cys136 and Cys155. Possible Implications in Pathophysiology. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32070004/ · DOI 10.3390/molecules25040820","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3a63272f-75b3-51fe-8aee-491afb50e72c","evidence_kind":"source_excerpt","locator":"Lines 146-152","start_line":146,"end_line":152,"excerpt":"## l-carnitine-gsh-carrier\nThe redox environment can change whether the carrier works.\nAdding reduced glutathione reversed copper inhibition of the reconstituted native rat carnitine-acylcarnitine carrier.\nModel: Rat-liver CACT proteoliposome assay.\nLimitations: Does not demonstrate oral glutathione-carnitine synergy.\nEvidence access: Primary abstract\nEffect of Copper on the Mitochondrial Carnitine/Acylcarnitine Carrier Via Interaction with Cys136 and Cys155. Possible Implications in Pathophysiology. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32070004/ · DOI 10.3390/molecules25040820","model_system":"Rat-liver CACT proteoliposome assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2c9c42b-806a-55e2-abde-b0320ea94704","stable_key":"import-6cdb37aa-8998-5ea8-829d-4f995caf98fc","title":"L-Carnitine: synthesis, acyl-group transport, fuel selection and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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