{"id":"943c0aa6-0d47-5d78-a950-2760de6992eb","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:nuclear-pkc","predicate":"context_dependent_observation","statement":"Zinc-thymulin increased nuclear PKC activity in isolated lymphocyte nuclei when IL-1 was present, but not without IL-1.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ecfa61f5-55e6-557e-af5d-79cf3458d0ad","mechanism_event_label":"Zinc-thymulin increased nuclear PKC activity in isolated lymphocyte nuclei when IL-1 was present, but not without IL-1.","subject":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"object":{"id":"3664e294-1e55-5994-a53c-79ec11602ed7","slug":"human-lymphocyte-nuclear-pkc","display_name":"Human lymphocyte nuclear PKC activity, isoform unresolved","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ecfa61f5-55e6-557e-af5d-79cf3458d0ad","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:nuclear-pkc-event","event_type":"experimental_observation","label":"Zinc-thymulin increased nuclear PKC activity in isolated lymphocyte nuclei when IL-1 was present, but not without IL-1.","description":"The human TEC study used induction of IL-2-receptor-positive lymphocytes as a functional readout. It also found that zinc–thymulin increased nuclear PKC activity in isolated lymphocyte nuclei **when IL-1 was present**, but not without IL-1. Keep the nuclear preparation, cytokine requirement and readout attached to that result. It does not establish an unconditional receptor → PKC → IL-2-production pathway in intact T cells. [Human-cell study](https://doi.org/10.1073/pnas.89.16.7752)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"role":"tested factor","stoichiometry":null,"state_label":"Zinc-thymulin with IL-1","sequence_order":0,"notes":""},{"entity":{"id":"3664e294-1e55-5994-a53c-79ec11602ed7","slug":"human-lymphocyte-nuclear-pkc","display_name":"Human lymphocyte nuclear PKC activity, isoform unresolved","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"mixed","sequence_order":1,"notes":""},{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"peptide-associated metal","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary bibliographic record and abstract checked; full methods/figures not comprehensively reviewed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"zinc-thymulin exposure","comparator":"Zinc-thymulin without IL-1","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Zinc-thymulin with IL-1\", \"comparator\": \"Zinc-thymulin without IL-1\", \"endpoint\": \"Zinc-thymulin increased nuclear PKC activity in isolated lymphocyte nuclei when IL-1 was present, but not without IL-1.\", \"effect_direction\": \"mixed\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"thymulin\", \"state\": \"zinc-thymulin exposure\"}, {\"entity_slug\": null, \"state\": \"IL-1 present\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Isolated human lymphocyte nuclei; cytokine-dependent effect, not unconditional intact-cell signaling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Zinc-thymulin increased nuclear PKC activity in isolated lymphocyte nuclei when IL-1 was present, but not without IL-1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Interleukin 1 regulates secretion of zinc-thymulin by human thymic epithelial cells and its action on T-lymphocyte proliferation and nuclear protein kinase C. | 1992 | DOI 10.1073/pnas.89.16.7752 | PMID 1502195 | https://doi.org/10.1073/pnas.89.16.7752","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 56-56; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"60700dc2-131c-5669-8d2a-998f6bc77d79","evidence_kind":"source_excerpt","locator":"Lines 56-56","start_line":56,"end_line":56,"excerpt":"The human TEC study used induction of IL-2-receptor-positive lymphocytes as a functional readout. It also found that zinc–thymulin increased nuclear PKC activity in isolated lymphocyte nuclei **when IL-1 was present**, but not without IL-1. Keep the nuclear preparation, cytokine requirement and readout attached to that result. It does not establish an unconditional receptor → PKC → IL-2-production pathway in intact T cells. [Human-cell study](https://doi.org/10.1073/pnas.89.16.7752)","model_system":"Isolated human lymphocyte nuclei; cytokine-dependent effect, not unconditional intact-cell signaling.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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