{"id":"93ee6503-f5f6-5816-b117-3aa0a477c61f","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-gaba-degradation","predicate":"consumes","statement":"Pig GABA aminotransferase contains a PLP-dependent active site for GABA degradation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"65a64854-c817-5c33-9fa2-fa452c0d96c2","mechanism_event_label":"B6 participates in GABA breakdown as well as synthesis.","subject":{"id":"4f496840-b2dd-5317-a6f5-72b2409b443b","slug":"pig-abat","display_name":"Pig GABA aminotransferase / ABAT","entity_type_key":"protein"},"object":{"id":"d5b98fa8-0612-5a5a-aa3c-6cfd8ff88867","slug":"gaba","display_name":"Gamma-aminobutyric acid","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"65a64854-c817-5c33-9fa2-fa452c0d96c2","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-gaba-degradation-event","event_type":"biochemical_relationship","label":"B6 participates in GABA breakdown as well as synthesis.","description":"Pig GABA aminotransferase contains a PLP-dependent active site for GABA degradation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4f496840-b2dd-5317-a6f5-72b2409b443b","slug":"pig-abat","display_name":"Pig GABA aminotransferase / ABAT","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d5b98fa8-0612-5a5a-aa3c-6cfd8ff88867","slug":"gaba","display_name":"Gamma-aminobutyric acid","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Purified pig GABA aminotransferase; crystallography","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Purified native pig GABA aminotransferase structure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pig enzyme evidence cannot alone predict human brain GABA responses to B6.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Sus scrofa","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"B6 participates in GABA breakdown as well as synthesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[storici-1999-gaba-at] Crystal structure of GABA-aminotransferase, a target for antiepileptic drug therapy (1999). https://iris.uniroma1.it/handle/11573/393739 DOI: 10.1021/bi990478j","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified pig enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"64ead22e-6900-5213-99af-8b436b67a350","evidence_kind":"source_excerpt","locator":"Lines 1071-1081","start_line":1071,"end_line":1081,"excerpt":"### b6-neuro-gaba-degradation\nPig GABA aminotransferase contains a PLP-dependent active site for GABA degradation.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: B6 participates in GABA breakdown as well as synthesis.\norganism: Sus scrofa\ntissue_or_cell_type: Purified pig enzyme\nexperimental_model: Purified pig GABA aminotransferase; crystallography\nlimitations: Pig enzyme evidence cannot alone predict human brain GABA responses to B6.\nexposure: Purified native pig GABA aminotransferase structure.\n[storici-1999-gaba-at] Crystal structure of GABA-aminotransferase, a target for antiepileptic drug therapy (1999). https://iris.uniroma1.it/handle/11573/393739 DOI: 10.1021/bi990478j","model_system":"Purified pig GABA aminotransferase; crystallography","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [storici-1999-gaba-at] Crystal structure of GABA-aminotransferase, a target for antiepileptic drug therapy (1999). https://iris.uniroma1.it/handle/11573/393739 DOI: 10.1021/bi990478j","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}