{"id":"93bcebf7-6d25-59b5-a250-293f7d1a894d","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-ligand-specific-sites","predicate":"discriminates_between","statement":"Human T1R2 amino-terminal-domain mutants showed ligand-specific and shared requirements across chemically different sweeteners.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"56fd8d2b-72ba-5e8e-9261-6d269a33600b","mechanism_event_label":"Aspartame and other sweeteners need not perturb every receptor contact equally.","subject":{"id":"09713aab-d5e3-589c-83c2-95d3989efd62","slug":"tas1r2","display_name":"Human taste receptor TAS1R2","entity_type_key":"protein"},"object":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"56fd8d2b-72ba-5e8e-9261-6d269a33600b","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-ligand-specific-sites-event","event_type":"observed_relationship","label":"Aspartame and other sweeteners need not perturb every receptor contact equally.","description":"Human T1R2 amino-terminal-domain mutants showed ligand-specific and shared requirements across chemically different sweeteners.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"09713aab-d5e3-589c-83c2-95d3989efd62","slug":"tas1r2","display_name":"Human taste receptor TAS1R2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7ab6f209-2089-5008-b64e-64f8db8c7771","slug":"tas1r3","display_name":"Human taste receptor TAS1R3","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Site-directed mutagenesis and docking.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A shared receptor is not proof that all sweeteners have interchangeable systemic effects.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Aspartame and other sweeteners need not perturb every receptor contact equally.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Characterization of the modes of binding between human sweet taste receptor and low-molecular-weight sweet compounds. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22536376/ · DOI 10.1371/journal.pone.0035380","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2c698726-6fab-5f3c-a055-c7d08c443f97","evidence_kind":"source_excerpt","locator":"Lines 186-192","start_line":186,"end_line":192,"excerpt":"## aspartame-ligand-specific-sites\nAspartame and other sweeteners need not perturb every receptor contact equally.\nHuman T1R2 amino-terminal-domain mutants showed ligand-specific and shared requirements across chemically different sweeteners.\nModel: Site-directed mutagenesis and docking.\nLimitations: A shared receptor is not proof that all sweeteners have interchangeable systemic effects.\nEvidence access: Primary abstract\nCharacterization of the modes of binding between human sweet taste receptor and low-molecular-weight sweet compounds. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22536376/ · DOI 10.1371/journal.pone.0035380","model_system":"Site-directed mutagenesis and docking.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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