{"id":"93893618-e564-56ad-8fc3-e4844622ec49","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-trp-autophagy","predicate":"deprivation_increases","statement":"Tryptophan deprivation increased LC3-based autophagy readouts in human HeLa cells; tryptophan or D-1-methyltryptophan reversed those readouts.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3be839f5-4b12-5023-b841-8bd3fcc69463","mechanism_event_label":"A shortage changed cellular recycling signals.","subject":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"object":{"id":"5af8c4a0-85a9-579b-b692-d6adc5f304a4","slug":"human-trp-autophagy","display_name":"Autophagic response to tryptophan deprivation in human cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3be839f5-4b12-5023-b841-8bd3fcc69463","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-trp-autophagy-event","event_type":"observed_relationship","label":"A shortage changed cellular recycling signals.","description":"Tryptophan deprivation increased LC3-based autophagy readouts in human HeLa cells; tryptophan or D-1-methyltryptophan reversed those readouts.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5af8c4a0-85a9-579b-b692-d6adc5f304a4","slug":"human-trp-autophagy","display_name":"Autophagic response to tryptophan deprivation in human cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"31060584-e38e-5f31-a742-de6e9a484faa","slug":"d-1-methyltryptophan","display_name":"D-1-Methyltryptophan / indoximod","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text, Figure 4","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human HeLa LC3 imaging and immunoblotting.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"LC3 measures do not establish every component of autophagic flux; the drug is not equivalent to nutrient replacement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A shortage changed cellular recycling signals.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"IDO inhibits a tryptophan sufficiency signal that stimulates mTOR: A novel IDO effector pathway targeted by D-1-methyl-tryptophan. · 2012 · https://pubmed.ncbi.nlm.nih.gov/23264892/ · DOI 10.4161/onci.21716","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"cd06e147-3f5f-511c-b45b-76bc79cfb71b","evidence_kind":"source_excerpt","locator":"Lines 410-416","start_line":410,"end_line":416,"excerpt":"## tryptophan-trp-autophagy\nA shortage changed cellular recycling signals.\nTryptophan deprivation increased LC3-based autophagy readouts in human HeLa cells; tryptophan or D-1-methyltryptophan reversed those readouts.\nModel: Human HeLa LC3 imaging and immunoblotting.\nLimitations: LC3 measures do not establish every component of autophagic flux; the drug is not equivalent to nutrient replacement.\nEvidence access: Primary full text, Figure 4\nIDO inhibits a tryptophan sufficiency signal that stimulates mTOR: A novel IDO effector pathway targeted by D-1-methyl-tryptophan. · 2012 · https://pubmed.ncbi.nlm.nih.gov/23264892/ · DOI 10.4161/onci.21716","model_system":"Human HeLa LC3 imaging and immunoblotting.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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