{"id":"93822985-b6ad-5f10-b81a-856e83814345","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:free-lps-jnk","predicate":"decreases_in_recorded_experiment","statement":"In repeated-LPS mice, free thymulin reduced reported splenic jnk phosphorylation/signaling readouts.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"f519a25d-13f3-54a7-93f3-2107e1a47a01","mechanism_event_label":"In repeated-LPS mice, free thymulin reduced reported splenic jnk phosphorylation/signaling readouts.","subject":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"object":{"id":"556ab115-21d6-55c5-97cd-adc37f22d371","slug":"mouse-jnk-assay-pool","display_name":"Mouse JNK assay pool, isoform unresolved","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"f519a25d-13f3-54a7-93f3-2107e1a47a01","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:free-lps-jnk-event","event_type":"experimental_observation","label":"In repeated-LPS mice, free thymulin reduced reported splenic jnk phosphorylation/signaling readouts.","description":"| Mice receiving repeated LPS, free thymulin or PBCA-bound thymulin | Reduced splenic phosphorylation of IKK, p65, JNK, p38 and PKCθ was reported, with changes in stress proteins and apoptosis-related readouts. | Repeated endotoxin exposure is not the same as human infectious sepsis. Free peptide and nanoparticle formulations require separate exposure records. [2018 experiment](https://doi.org/10.1371/journal.pone.0197601) |","status":"provisional","compartment":null,"participants":[{"entity":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"role":"tested factor","stoichiometry":null,"state_label":"thymulin with repeated LPS","sequence_order":0,"notes":""},{"entity":{"id":"556ab115-21d6-55c5-97cd-adc37f22d371","slug":"mouse-jnk-assay-pool","display_name":"Mouse JNK assay pool, isoform unresolved","entity_type_key":"protein"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Full text obtained; relevant results/methods inspected. 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Free and nanoparticle formulations are separate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"In repeated-LPS mice, free thymulin reduced reported splenic jnk phosphorylation/signaling readouts.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Thymulin, free or bound to PBCA nanoparticles, protects mice against chronic septic inflammation | 2018 | DOI 10.1371/journal.pone.0197601 | PMID 29795607 | https://doi.org/10.1371/journal.pone.0197601","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 71-71; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"59fc73bb-30fa-5e13-a33d-f2f1fd604c44","evidence_kind":"source_excerpt","locator":"Lines 71-71","start_line":71,"end_line":71,"excerpt":"| Mice receiving repeated LPS, free thymulin or PBCA-bound thymulin | Reduced splenic phosphorylation of IKK, p65, JNK, p38 and PKCθ was reported, with changes in stress proteins and apoptosis-related readouts. | Repeated endotoxin exposure is not the same as human infectious sepsis. Free peptide and nanoparticle formulations require separate exposure records. [2018 experiment](https://doi.org/10.1371/journal.pone.0197601) |","model_system":"Repeated-LPS mouse model; not human infectious sepsis. Free and nanoparticle formulations are separate.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e1871d49-9c6b-547b-a1bb-6e2b9270ca89","stable_key":"import-18100170-a014-51c3-adeb-9dab4162b8cc","title":"Thymulin: detailed mechanisms of action (reviewed 3 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. 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