{"id":"935f999a-15a1-583a-b90d-86a229f245c2","stable_key":"c932089b-ebc9-5f39-b2e5-84af74f9f6d0:sucrose-biofilm-matrix","predicate":"supports","statement":"Sucrose-supported multispecies cultures formed a glucan matrix and structured microcolonies; glucose cultures did not form that matrix under the tested conditions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"229ce21a-7e9c-5a41-a04f-03cbdb9a490b","mechanism_event_label":"Sucrose-supported multispecies cultures formed a glucan matrix and structured microcolonies; glucose cultures did not form that matrix under the tested conditions.","subject":{"id":"82558bd0-1a84-574a-a04c-10609c9c5dba","slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"},"object":{"id":"0d89cdcb-ac32-5c93-8412-750085b62aef","slug":"streptococcus-mutans-extracellular-glucan-matrix","display_name":"Streptococcus mutans extracellular glucan matrix formation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"229ce21a-7e9c-5a41-a04f-03cbdb9a490b","stable_key":"c932089b-ebc9-5f39-b2e5-84af74f9f6d0:sucrose-biofilm-matrix-event","event_type":"observed_relationship","label":"Sucrose-supported multispecies cultures formed a glucan matrix and structured microcolonies; glucose cultures did not form that matrix under the tested conditions.","description":"Sucrose-supported multispecies cultures formed a glucan matrix and structured microcolonies; glucose cultures did not form that matrix under the tested conditions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"82558bd0-1a84-574a-a04c-10609c9c5dba","slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0d89cdcb-ac32-5c93-8412-750085b62aef","slug":"streptococcus-mutans-extracellular-glucan-matrix","display_name":"Streptococcus mutans extracellular glucan matrix formation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"98e9eb93-6ef0-57b6-9122-e41847a99fe3","slug":"streptococcus-mutans-gtfb","display_name":"Streptococcus mutans glucosyltransferase B / GtfB","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"fe45c655-bd6f-5f63-97d9-67ad77fc1320","slug":"streptococcus-mutans-gtfc","display_name":"Streptococcus mutans glucosyltransferase C / GtfC","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"e00be64b-622f-5da8-bda3-27924063c497","slug":"streptococcus-mutans-biofilm-microcolonies","display_name":"Streptococcus mutans biofilm microcolony formation","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Sucrose versus glucose growth conditions; gtfB or gtfB/gtfC deletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Biofilm maturation series; exact dose and duration not recovered from accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract/metadata; unrecovered methods explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Streptococcus mutans with Streptococcus oralis and Actinomyces naeslundii; gtf mutants","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_scope","value_text":"Sucrose-dependent oral bacterial machinery","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Bacterial matrix formation and mutant phenotypes; not a clinical caries incidence trial. GtfB and GtfC have different contributions. Only primary abstract and metadata used.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sucrose chapter; direct sucrose observations are distinguished from shared component metabolism.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Streptococcus mutans with Streptococcus oralis and Actinomyces naeslundii; gtf mutants","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Sucrose-supported multispecies cultures formed a glucan matrix and structured microcolonies; glucose cultures did not form that matrix under the tested conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Exopolysaccharides produced by Streptococcus mutans glucosyltransferases modulate the establishment of microcolonies within multispecies biofilms. (2010). https://pubmed.ncbi.nlm.nih.gov/20233920/ DOI: 10.1128/JB.01649-09","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro bacterial culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Biofilm on saliva-coated hydroxyapatite","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c947441e-99a1-5330-a751-7f457bd654d5","evidence_kind":"source_excerpt","locator":"Lines 403-413","start_line":403,"end_line":413,"excerpt":"## sucrose-biofilm-matrix\nSucrose-supported multispecies cultures formed a glucan matrix and structured microcolonies; glucose cultures did not form that matrix under the tested conditions.\nModel/species: Streptococcus mutans with Streptococcus oralis and Actinomyces naeslundii; gtf mutants\nTissue: Biofilm on saliva-coated hydroxyapatite\nExposure: Sucrose versus glucose growth conditions; gtfB or gtfB/gtfC deletion\nRoute: In vitro bacterial culture\nDuration: Biofilm maturation series; exact dose and duration not recovered from accessed abstract\nExposure scope: Sucrose-dependent oral bacterial machinery\nLimits: Bacterial matrix formation and mutant phenotypes; not a clinical caries incidence trial. GtfB and GtfC have different contributions. Only primary abstract and metadata used.\nReference: Exopolysaccharides produced by Streptococcus mutans glucosyltransferases modulate the establishment of microcolonies within multispecies biofilms. (2010). https://pubmed.ncbi.nlm.nih.gov/20233920/ DOI: 10.1128/JB.01649-09\nAccess: Primary abstract/metadata; unrecovered methods explicitly retained.","model_system":"Streptococcus mutans with Streptococcus oralis and Actinomyces naeslundii; gtf mutants","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; provenance retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"8e24ddf4-84ee-57b4-9fd8-093547432ece","stable_key":"import-c932089b-ebc9-5f39-b2e5-84af74f9f6d0","title":"Sucrose: mechanism of action and metabolic impact (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific sucrose curation with shared canonical claims retained by identity. Primary-study citations, negative findings, exposure details and limitations preserved. 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