{"id":"9338a473-c9fa-5936-a584-894d8a616cfa","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-oxygen-hif","predicate":"modulates","statement":"Butyrate increased epithelial oxygen consumption and stabilized HIF in intestinal epithelial-cell experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6820338d-cfbf-5fac-abbb-05ce36625040","mechanism_event_label":"Using the fuel changed local oxygen sensing.","subject":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"object":{"id":"32fa1079-7b37-5178-8fbb-ae1597ea1198","slug":"human-butyrate-hif-stabilization","display_name":"HIF stabilization in butyrate-exposed human epithelial cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6820338d-cfbf-5fac-abbb-05ce36625040","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-oxygen-hif-event","event_type":"observed_relationship","label":"Using the fuel changed local oxygen sensing.","description":"Butyrate increased epithelial oxygen consumption and stabilized HIF in intestinal epithelial-cell experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"32fa1079-7b37-5178-8fbb-ae1597ea1198","slug":"human-butyrate-hif-stabilization","display_name":"HIF stabilization in butyrate-exposed human epithelial cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"22b3e241-94b0-55fd-a233-c63af92520d8","slug":"hif1a","display_name":"HIF-1 alpha","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human intestinal epithelial cultures, with separate mouse depletion/repletion experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"HIF stabilization has more than one possible route; this result does not exclude direct hydroxylase inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Butyrate collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Using the fuel changed local oxygen sensing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Crosstalk between Microbiota-Derived Short-Chain Fatty Acids and Intestinal Epithelial HIF Augments Tissue Barrier Function. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25865369/ · DOI 10.1016/j.chom.2015.03.005","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"88a8ca54-7df9-5219-a82c-ebdb590af331","evidence_kind":"source_excerpt","locator":"Lines 238-244","start_line":238,"end_line":244,"excerpt":"## butyrate-oxygen-hif\nUsing the fuel changed local oxygen sensing.\nButyrate increased epithelial oxygen consumption and stabilized HIF in intestinal epithelial-cell experiments.\nModel: Human intestinal epithelial cultures, with separate mouse depletion/repletion experiments.\nLimitations: HIF stabilization has more than one possible route; this result does not exclude direct hydroxylase inhibition.\nEvidence access: Primary abstract\nCrosstalk between Microbiota-Derived Short-Chain Fatty Acids and Intestinal Epithelial HIF Augments Tissue Barrier Function. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25865369/ · DOI 10.1016/j.chom.2015.03.005","model_system":"Human intestinal epithelial cultures, with separate mouse depletion/repletion experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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