{"id":"93315bce-fb37-5002-b311-de09ae229112","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-hlcs-acacb-acceptor","predicate":"biotinylates","statement":"HLCS biotinylation of the human ACACB acceptor fragment was relatively slow and depended hyperbolically on fragment concentration.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"bda9ae29-4d6a-58d8-9f5b-7ba03a063485","mechanism_event_label":"The two acetyl-CoA carboxylases showed different attachment kinetics from the mitochondrial carboxylase fragments.","subject":{"id":"518c7af6-7e78-59ed-a9cb-3de53c9a39c8","slug":"hlcs","display_name":"Human holocarboxylase synthetase / HLCS","entity_type_key":"protein"},"object":{"id":"c9808f71-628e-52db-989a-53f6f128e78c","slug":"acacb","display_name":"Human acetyl-CoA carboxylase 2 / ACACB","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"bda9ae29-4d6a-58d8-9f5b-7ba03a063485","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-hlcs-acacb-acceptor-event","event_type":"biochemical_relationship","label":"The two acetyl-CoA carboxylases showed different attachment kinetics from the mitochondrial carboxylase fragments.","description":"HLCS biotinylation of the human ACACB acceptor fragment was relatively slow and depended hyperbolically on fragment concentration.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"518c7af6-7e78-59ed-a9cb-3de53c9a39c8","slug":"hlcs","display_name":"Human holocarboxylase synthetase / HLCS","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c9808f71-628e-52db-989a-53f6f128e78c","slug":"acacb","display_name":"Human acetyl-CoA carboxylase 2 / ACACB","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/biotin-research/22123817.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"559afe20b6f8601a2d055d42fb121c89eaf1ece76afaa5cc0817c20787893233\", \"start_char\": 0, \"end_char\": 1502, \"text_sha256\": \"559afe20b6f8601a2d055d42fb121c89eaf1ece76afaa5cc0817c20787893233\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Single-turnover biotin transfer to minimal BCCP fragments from all five human carboxylases","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Stopped-flow and quench-flow transfer kinetics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Fragment kinetics support differential recognition, not a universal nutrient-deficiency survival order in humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Biotin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The two acetyl-CoA carboxylases showed different attachment kinetics from the mitochondrial carboxylase fragments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b7-p22123817] Selectivity in post-translational biotin addition to five human carboxylases. 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