{"id":"932af280-c87b-53c5-af15-6e96da5987fc","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-bdnf-knockdown","predicate":"knockdown_blocks","statement":"Hippocampal Bdnf siRNA blocked the memory-enabling effect of post-training sodium butyrate in the mouse task.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"77355192-96d7-566c-83fb-496a52ba9f13","mechanism_event_label":"Reducing a neural growth-factor signal removed the measured benefit.","subject":{"id":"8d4e2cbf-5be6-54a1-8b6e-5195fe680373","slug":"mouse-bdnf","display_name":"Mouse brain-derived neurotrophic factor / Bdnf","entity_type_key":"protein"},"object":{"id":"3bf6d352-83be-5bba-8a28-168a758bc39a","slug":"mouse-butyrate-memory","display_name":"Mouse object-location memory after injected sodium butyrate","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"77355192-96d7-566c-83fb-496a52ba9f13","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-bdnf-knockdown-event","event_type":"observed_relationship","label":"Reducing a neural growth-factor signal removed the measured benefit.","description":"Hippocampal Bdnf siRNA blocked the memory-enabling effect of post-training sodium butyrate in the mouse task.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8d4e2cbf-5be6-54a1-8b6e-5195fe680373","slug":"mouse-bdnf","display_name":"Mouse brain-derived neurotrophic factor / Bdnf","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3bf6d352-83be-5bba-8a28-168a758bc39a","slug":"mouse-butyrate-memory","display_name":"Mouse object-location memory after injected sodium butyrate","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4baf6011-dcc4-5a69-9c77-a21de6060383","slug":"sodium-butyrate","display_name":"Sodium butyrate","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse hippocampal siRNA and object-location memory assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Task- and intervention-specific; not a universal mechanism for every memory effect of butyrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Butyrate collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Reducing a neural growth-factor signal removed the measured benefit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Exercise and sodium butyrate transform a subthreshold learning event into long-term memory via a brain-derived neurotrophic factor-dependent mechanism. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23615664/ · DOI 10.1038/npp.2013.104","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"f55460d0-462f-531e-a8bd-9136c63168ae","evidence_kind":"source_excerpt","locator":"Lines 630-636","start_line":630,"end_line":636,"excerpt":"## butyrate-bdnf-knockdown\nReducing a neural growth-factor signal removed the measured benefit.\nHippocampal Bdnf siRNA blocked the memory-enabling effect of post-training sodium butyrate in the mouse task.\nModel: Mouse hippocampal siRNA and object-location memory assay.\nLimitations: Task- and intervention-specific; not a universal mechanism for every memory effect of butyrate.\nEvidence access: Primary abstract\nExercise and sodium butyrate transform a subthreshold learning event into long-term memory via a brain-derived neurotrophic factor-dependent mechanism. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23615664/ · DOI 10.1038/npp.2013.104","model_system":"Mouse hippocampal siRNA and object-location memory assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"a1253eb4426fc45d7b7c113028aa4ba2e9f89a9653272f3795437fc222bad558","revision_id":"c2320e65-a252-57a9-8838-7abf3d23400e","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}