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(2013). https://pubmed.ncbi.nlm.nih.gov/23812591/ DOI: 10.1038/nature12362","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Embryonic stem cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"db39e6ec-d20f-554c-89e7-6fa7656593b7","evidence_kind":"source_excerpt","locator":"Lines 977-989","start_line":977,"end_line":989,"excerpt":"### c-reg-esc-hydroxymethylation\nAdding L-ascorbic acid 2-phosphate to mouse ESC medium rapidly increased global DNA 5hmC and promoter-associated 5hmC, preceding later methylcytosine loss.\nCondition category: normal\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A stable vitamin C derivative increased an early DNA-demethylation intermediate in cultured mouse stem cells.\norganism: Mus musculus\ntissue_or_cell_type: Embryonic stem cells\nexperimental_model: Mouse Oct4-GiP ESCs in feeder-free 2i/LIF medium; DNA dot blot and hydroxymethyl-DNA immunoprecipitation\nlimitations: 5hmC abundance alone is not proof of completed demethylation; cultured ESCs are not adult human tissues.\nexposure: 100 µg/mL L-ascorbic acid 2-phosphate (A8960), medium replaced daily; 12-h and 72-h sampling.\ncross_nutrient: false\nevidence_location: Figures 1–2\n[c-reg-blaschke] Vitamin C induces Tet-dependent DNA demethylation and a blastocyst-like state in ES cells. 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