{"id":"92fe997f-6f6a-5b90-8c30-949f2b3c4e13","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-microbial-zinc","predicate":"inhibits","statement":"Zinc strongly inhibited purified C. difficile D-proline reductase, which did not require added divalent cations for its measured activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"8654f070-ff5b-542c-8cfe-12a539c2a26c","mechanism_event_label":"A mineral can inhibit this microbial enzyme rather than serve as its cofactor.","subject":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"object":{"id":"e6885988-faae-5f4f-972b-9931b65d5e17","slug":"c-difficile-proline-reductase","display_name":"Clostridioides difficile D-proline reductase complex","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"8654f070-ff5b-542c-8cfe-12a539c2a26c","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-microbial-zinc-event","event_type":"observed_relationship","label":"A mineral can inhibit this microbial enzyme rather than serve as its cofactor.","description":"Zinc strongly inhibited purified C. difficile D-proline reductase, which did not require added divalent cations for its measured activity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e6885988-faae-5f4f-972b-9931b65d5e17","slug":"c-difficile-proline-reductase","display_name":"Clostridioides difficile D-proline reductase complex","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6459a2bf-7e42-5037-a0b1-51d72a0c5845","slug":"d-proline","display_name":"D-Proline","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified bacterial-enzyme assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No safe or effective intestinal zinc intervention is established by the enzyme experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Proline collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A mineral can inhibit this microbial enzyme rather than serve as its cofactor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Analysis of proline reduction in the nosocomial pathogen Clostridium difficile. · 2006 · https://pubmed.ncbi.nlm.nih.gov/17041035/ · DOI 10.1128/JB.01370-06","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"20e7aabc-3dc4-54e1-b0b7-40d35407fde4","evidence_kind":"source_excerpt","locator":"Lines 422-428","start_line":422,"end_line":428,"excerpt":"## l-proline-microbial-zinc\nA mineral can inhibit this microbial enzyme rather than serve as its cofactor.\nZinc strongly inhibited purified C. difficile D-proline reductase, which did not require added divalent cations for its measured activity.\nModel: Purified bacterial-enzyme assays.\nLimitations: No safe or effective intestinal zinc intervention is established by the enzyme experiment.\nEvidence access: Primary abstract\nAnalysis of proline reduction in the nosocomial pathogen Clostridium difficile. · 2006 · https://pubmed.ncbi.nlm.nih.gov/17041035/ · DOI 10.1128/JB.01370-06","model_system":"Purified bacterial-enzyme assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e5aa7fc5-ee52-5376-8169-416082a89fd1","stable_key":"import-6612c190-1948-5bcf-bbe3-a7f6c50fa3cf","title":"L-Proline: synthesis, collagen processing, redox metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7b7f1981f9c7897fbb6baa19425bdc19dede78ad4d66fa554394b8337c7366fb","revision_id":"4971a925-fa70-59f8-8030-d3029a18a62f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}