{"id":"92eb7454-13ae-566b-9a0a-118a1633d81f","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-microsome-glucuronidation","predicate":"reported_relationship","statement":"Human liver microsomes generated RA glucuronides, including the major reported M8 pathway.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"6a3b435b-0e46-5e09-92d6-4000b324f975","mechanism_event_label":"Human liver microsomes generated RA glucuronides, including the major reported M8 pathway.","subject":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"object":{"id":"deeedfee-f9b7-5c81-aea4-8097d4307aa9","slug":"ra-glucuronide-metabolites","display_name":"Rosmarinic-acid glucuronide metabolites, isomers explicit in source","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"6a3b435b-0e46-5e09-92d6-4000b324f975","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-microsome-glucuronidation-event","event_type":"biochemical_relationship","label":"Human liver microsomes generated RA glucuronides, including the major reported M8 pathway.","description":"### ra-microsome-glucuronidation\n\nHuman liver microsomes generated RA glucuronides, including the major reported M8 pathway.\nMetabolites assigned by high-resolution LC-MS/MS.\nModel and exposure: Microsomal incubation, 60 min; cofactor and GSH trapping conditions.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Characterization of the metabolites of rosmarinic acid in human liver microsomes using liquid chromatography combined with electrospray ionization tandem mass spectrometry. | 2020 | https://pubmed.ncbi.nlm.nih.gov/32012312/ | https://doi.org/10.1002/bmc.4806 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"role":"recorded factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"deeedfee-f9b7-5c81-aea4-8097d4307aa9","slug":"ra-glucuronide-metabolites","display_name":"Rosmarinic-acid glucuronide metabolites, isomers explicit in source","entity_type_key":"chemical_species"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""},{"entity":{"id":"fea4f3f0-7c05-59a2-9332-445b9d4f8fb0","slug":"udp-glucuronic-acid","display_name":"UDP-glucuronic acid","entity_type_key":"small_molecule"},"role":"glucuronide donor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Microsomal incubation, 60 min; cofactor and GSH trapping conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Rosmarinic acid collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Human liver microsomes generated RA glucuronides, including the major reported M8 pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Characterization of the metabolites of rosmarinic acid in human liver microsomes using liquid chromatography combined with electrospray ionization tandem mass spectrometry. | https://pubmed.ncbi.nlm.nih.gov/32012312/ | https://doi.org/10.1002/bmc.4806","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference ra-microsome-glucuronidation; Metabolites assigned by high-resolution LC-MS/MS.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"35712e7f-3ba5-593b-a066-42a3b21599c3","evidence_kind":"source_excerpt","locator":"Lines 137-143","start_line":137,"end_line":143,"excerpt":"### ra-microsome-glucuronidation\n\nHuman liver microsomes generated RA glucuronides, including the major reported M8 pathway.\nMetabolites assigned by high-resolution LC-MS/MS.\nModel and exposure: Microsomal incubation, 60 min; cofactor and GSH trapping conditions.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Characterization of the metabolites of rosmarinic acid in human liver microsomes using liquid chromatography combined with electrospray ionization tandem mass spectrometry. | 2020 | https://pubmed.ncbi.nlm.nih.gov/32012312/ | https://doi.org/10.1002/bmc.4806 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"Microsomal incubation, 60 min; cofactor and GSH trapping conditions.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"606cff22-a6ed-59cd-a84d-87f44c59dc25","stable_key":"import-ca8e9e6a-6953-51ec-b712-2b06398a416d","title":"Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"684b4eab1dc2c0057f679109770af0de9340af5cd933377b590298cf8a752956","revision_id":"4028d9c5-fb17-54c5-b72c-c6d21678a832","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}