{"id":"92dfe5f1-9926-59f9-bccb-ec14acfabf84","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:calcium-gh","predicate":"decreases_in_recorded_experiment","statement":"EGTA blocked thymulin-stimulated GH release in dispersed rat pituitary preparations; cAMP enhancers potentiated the response.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"f3fb3573-a707-554c-ab90-28cfeaf2bcd1","mechanism_event_label":"EGTA blocked thymulin-stimulated GH release in dispersed rat pituitary preparations; cAMP enhancers potentiated the response.","subject":{"id":"bb75c16e-757d-5817-b998-dfe2989f4013","slug":"egta","display_name":"EGTA","entity_type_key":"small_molecule"},"object":{"id":"93f426ba-6772-5feb-974b-9f045ca73979","slug":"rat-pituitary-gh-release","display_name":"Rat anterior-pituitary growth-hormone release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f3fb3573-a707-554c-ab90-28cfeaf2bcd1","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:calcium-gh-event","event_type":"experimental_observation","label":"EGTA blocked thymulin-stimulated GH release in dispersed rat pituitary preparations; cAMP enhancers potentiated the response.","description":"In a different dispersed-rat-pituitary study using higher concentrations, thymulin stimulated **GH** release; responses depended on age and concentration. EGTA blocked activity, and cAMP enhancers potentiated it. These interventions support calcium-sensitive signaling, not a requirement for calcium bound directly to thymulin. The earlier GH null remains part of the evidence. [1999 GH study](https://pubmed.ncbi.nlm.nih.gov/9892847/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bb75c16e-757d-5817-b998-dfe2989f4013","slug":"egta","display_name":"EGTA","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"EGTA plus thymulin","sequence_order":0,"notes":""},{"entity":{"id":"93f426ba-6772-5feb-974b-9f045ca73979","slug":"rat-pituitary-gh-release","display_name":"Rat anterior-pituitary growth-hormone release","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"chelated signaling ion","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"role":"joint hormone exposure","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary bibliographic record and abstract checked; full methods/figures not comprehensively reviewed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"chelation","comparator":"Thymulin without EGTA","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"egta","display_name":"EGTA","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"hormone exposure","comparator":"Thymulin without EGTA","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"EGTA plus thymulin\", \"comparator\": \"Thymulin without EGTA\", \"endpoint\": \"EGTA blocked thymulin-stimulated GH release in dispersed rat pituitary preparations; cAMP enhancers potentiated the response.\", \"effect_direction\": \"decrease\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"egta\", \"state\": \"chelation\"}, {\"entity_slug\": \"thymulin\", \"state\": \"hormone exposure\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Rat pituitary preparations; extracellular calcium chelation supports calcium-sensitive signaling, not direct calcium binding to thymulin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"EGTA blocked thymulin-stimulated GH release in dispersed rat pituitary preparations; cAMP enhancers potentiated the response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Growth hormone-releasing activity of thymulin on pituitary somatotropes is age dependent. | 1999 | DOI 10.1159/000054399 | PMID 9892847 | https://pubmed.ncbi.nlm.nih.gov/9892847/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 106-106; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a322d224-4145-5103-a5b4-4a358fd9f5a4","evidence_kind":"source_excerpt","locator":"Lines 106-106","start_line":106,"end_line":106,"excerpt":"In a different dispersed-rat-pituitary study using higher concentrations, thymulin stimulated **GH** release; responses depended on age and concentration. EGTA blocked activity, and cAMP enhancers potentiated it. These interventions support calcium-sensitive signaling, not a requirement for calcium bound directly to thymulin. The earlier GH null remains part of the evidence. [1999 GH study](https://pubmed.ncbi.nlm.nih.gov/9892847/)","model_system":"Rat pituitary preparations; extracellular calcium chelation supports calcium-sensitive signaling, not direct calcium binding to thymulin.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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