{"id":"92d852c8-4999-5b75-b34a-2030b11ad483","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:opg-binds-rankl","predicate":"binds","statement":"Osteoprotegerin bound RANKL/ODF and blocked its osteoclastogenic activity in the mouse culture system.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"3e0e4bdd-8890-556c-aa75-485cba5328e0","mechanism_event_label":"OPG can intercept the signal that promotes bone-resorbing cells.","subject":{"id":"02f7fab4-c764-5448-bf91-7b5f6e5f76e5","slug":"tnfrsf11b","display_name":"Osteoprotegerin / TNFRSF11B / OPG","entity_type_key":"protein"},"object":{"id":"de7ad036-388f-596d-8766-72afe51b4a67","slug":"tnfsf11","display_name":"RANK ligand / TNFSF11","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"3e0e4bdd-8890-556c-aa75-485cba5328e0","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:opg-binds-rankl-event","event_type":"biochemical_relationship","label":"OPG can intercept the signal that promotes bone-resorbing cells.","description":"Osteoprotegerin bound RANKL/ODF and blocked its osteoclastogenic activity in the mouse culture system.","status":"provisional","compartment":{"slug":"extracellular-space","display_name":"Extracellular space"},"participants":[{"entity":{"id":"02f7fab4-c764-5448-bf91-7b5f6e5f76e5","slug":"tnfrsf11b","display_name":"Osteoprotegerin / TNFRSF11B / OPG","entity_type_key":"protein"},"role":"soluble_decoy_receptor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"de7ad036-388f-596d-8766-72afe51b4a67","slug":"tnfsf11","display_name":"RANK ligand / TNFSF11","entity_type_key":"protein"},"role":"bound_ligand","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a4c1aaa0-3366-5bb2-bc5c-910241d8b64b","slug":"osteoclast-differentiation","display_name":"Osteoclast differentiation","entity_type_key":"cellular_process"},"role":"inhibited_process","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"3dcbbcaa-17df-54a0-a499-9b966a38631a","slug":"extracellular-space","display_name":"Extracellular space","entity_type_key":"organelle"},"role":"experimental_location","stoichiometry":null,"state_label":"","sequence_order":3,"notes":"Study location; presence here is not a separate transport or causal claim."}]},"contexts":[{"dimension":"compartment_description","value_text":"Extracellular space","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Ligand identification and inhibition assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The experiment establishes ligand sequestration in this system, not clinical fracture protection.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Calcium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus cell system","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"OPG can intercept the signal that promotes bone-resorbing cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[yasuda1998] Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL (1998). https://pmc.ncbi.nlm.nih.gov/articles/PMC19881/ DOI: 10.1073/pnas.95.7.3597","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Stromal and osteoclast-precursor cultures","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"98752087-8c78-503a-80db-b6b48677c6ff","evidence_kind":"source_excerpt","locator":"Lines 1047-1057","start_line":1047,"end_line":1057,"excerpt":"### opg-binds-rankl\nOsteoprotegerin bound RANKL/ODF and blocked its osteoclastogenic activity in the mouse culture system.\nCondition category: normal\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: OPG can intercept the signal that promotes bone-resorbing cells.\norganism: Mus musculus cell system\ntissue_or_cell_type: Stromal and osteoclast-precursor cultures\nexperimental_model: Ligand identification and inhibition assays\nlimitations: The experiment establishes ligand sequestration in this system, not clinical fracture protection.\ncompartment_description: Extracellular space\n[yasuda1998] Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL (1998). https://pmc.ncbi.nlm.nih.gov/articles/PMC19881/ DOI: 10.1073/pnas.95.7.3597","model_system":"Ligand identification and inhibition assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [yasuda1998] Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL (1998). https://pmc.ncbi.nlm.nih.gov/articles/PMC19881/ DOI: 10.1073/pnas.95.7.3597","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}