{"id":"927e4825-5cdd-5362-ab2c-d2be24cc6df7","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:resting-rat","predicate":"decreases_in_recorded_experiment","statement":"Resting crushed garlic before microwave heating preserved some inhibition of rat mammary carcinogen-DNA adduct formation.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"492c7a94-c2ff-5ac4-b4da-1ffb2d2d766f","mechanism_event_label":"Resting crushed garlic before microwave heating preserved some inhibition of rat mammary carcinogen-DNA adduct formation.","subject":{"id":"f33785be-a1a9-516d-be69-f03b8dfcb99b","slug":"garlic-heated-song-2001","display_name":"Garlic preparations in the rat mammary DNA-adduct heating study","entity_type_key":"botanical"},"object":{"id":"bcc2c7dc-e9df-5344-aa9a-6832c482c217","slug":"rat-mammary-dmba-dna-adducts","display_name":"DMBA-metabolite DNA adducts in rat mammary epithelium","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"492c7a94-c2ff-5ac4-b4da-1ffb2d2d766f","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:resting-rat-event","event_type":"experimental_observation","label":"Resting crushed garlic before microwave heating preserved some inhibition of rat mammary carcinogen-DNA adduct formation.","description":"**The ten-minute rest.** A rat mammary DNA-adduct experiment found that letting crushed garlic stand ten minutes before a sixty-second microwave exposure prevented complete loss of the measured protective effect. The endpoint involved carcinogen-metabolite binding to DNA. It neither establishes a universal culinary optimum nor proves prevention of human cancer. [Song and Milner 2001](https://pubmed.ncbi.nlm.nih.gov/11238815/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f33785be-a1a9-516d-be69-f03b8dfcb99b","slug":"garlic-heated-song-2001","display_name":"Garlic preparations in the rat mammary DNA-adduct heating study","entity_type_key":"botanical"},"role":"tested factor","stoichiometry":null,"state_label":"Crushed garlic rested before heating","sequence_order":0,"notes":""},{"entity":{"id":"bcc2c7dc-e9df-5344-aa9a-6832c482c217","slug":"rat-mammary-dmba-dna-adducts","display_name":"DMBA-metabolite DNA adducts in rat mammary epithelium","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full methods, exact concentrations or species-specific attribution remain unextracted unless explicitly stated in the abstract or separately verified publisher text.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Crushed garlic rested before heating\", \"comparator\": \"Garlic heated without the preheating rest\", \"endpoint\": \"Resting crushed garlic before microwave heating preserved some inhibition of rat mammary carcinogen-DNA adduct formation.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Rat mammary DMBA adduct endpoint; ten-minute rest before sixty-second microwave exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Preparation-specific animal surrogate endpoint, not human cancer prevention.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Resting crushed garlic before microwave heating preserved some inhibition of rat mammary carcinogen-DNA adduct formation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The influence of heating on the anticancer properties of garlic. | 2001 | DOI 10.1093/jn/131.3.1054s | PMID 11238815 | https://pubmed.ncbi.nlm.nih.gov/11238815/ | https://doi.org/10.1093/jn/131.3.1054s","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 23-23; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"50d19f87-5c0f-5fb4-9940-a78584c42381","evidence_kind":"source_excerpt","locator":"Lines 23-23","start_line":23,"end_line":23,"excerpt":"**The ten-minute rest.** A rat mammary DNA-adduct experiment found that letting crushed garlic stand ten minutes before a sixty-second microwave exposure prevented complete loss of the measured protective effect. The endpoint involved carcinogen-metabolite binding to DNA. It neither establishes a universal culinary optimum nor proves prevention of human cancer. [Song and Milner 2001](https://pubmed.ncbi.nlm.nih.gov/11238815/)","model_system":"Rat mammary DMBA adduct endpoint; ten-minute rest before sixty-second microwave exposure.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. 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