{"id":"92663267-0c90-50a5-b380-dc22ec2168ef","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-vigabatrin-plp-adduct","predicate":"inactivates","statement":"Vigabatrin formed a covalent adduct involving pig GABA-AT Lys329 and its PLP cofactor.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"d4966f93-2f0f-5e02-ab54-75a8201d2c20","mechanism_event_label":"The drug traps the enzyme together with its B6 cofactor.","subject":{"id":"694d1817-618d-5b0f-9222-cccdf2a43b31","slug":"vigabatrin","display_name":"Vigabatrin","entity_type_key":"small_molecule"},"object":{"id":"4f496840-b2dd-5317-a6f5-72b2409b443b","slug":"pig-abat","display_name":"Pig GABA aminotransferase / ABAT","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"d4966f93-2f0f-5e02-ab54-75a8201d2c20","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-vigabatrin-plp-adduct-event","event_type":"biochemical_relationship","label":"The drug traps the enzyme together with its B6 cofactor.","description":"Vigabatrin formed a covalent adduct involving pig GABA-AT Lys329 and its PLP cofactor.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"694d1817-618d-5b0f-9222-cccdf2a43b31","slug":"vigabatrin","display_name":"Vigabatrin","entity_type_key":"small_molecule"},"role":"inhibitor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4f496840-b2dd-5317-a6f5-72b2409b443b","slug":"pig-abat","display_name":"Pig GABA aminotransferase / ABAT","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"adduct-partner","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Pig liver GABA aminotransferase; inhibitor complexes and spectroscopy","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Inhibitor-treated purified enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not establish systemic B6 depletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Sus scrofa","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The drug traps the enzyme together with its B6 cofactor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[storici-2004-gaba-at] Structures of gamma-aminobutyric acid (GABA) aminotransferase, a pyridoxal 5'-phosphate, and [2Fe-2S] cluster-containing enzyme, complexed with gamma-ethynyl-GABA and with the antiepilepsy drug vigabatrin (2004). https://doi.org/10.1074/jbc.M305884200 DOI: 10.1074/jbc.M305884200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Pig liver enzyme","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"ac492c00-23d9-524f-9c21-e238ee28def9","evidence_kind":"source_excerpt","locator":"Lines 1096-1106","start_line":1096,"end_line":1106,"excerpt":"### b6-neuro-vigabatrin-plp-adduct\nVigabatrin formed a covalent adduct involving pig GABA-AT Lys329 and its PLP cofactor.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The drug traps the enzyme together with its B6 cofactor.\norganism: Sus scrofa\ntissue_or_cell_type: Pig liver enzyme\nexperimental_model: Pig liver GABA aminotransferase; inhibitor complexes and spectroscopy\nlimitations: Does not establish systemic B6 depletion.\nexposure: Inhibitor-treated purified enzyme.\n[storici-2004-gaba-at] Structures of gamma-aminobutyric acid (GABA) aminotransferase, a pyridoxal 5'-phosphate, and [2Fe-2S] cluster-containing enzyme, complexed with gamma-ethynyl-GABA and with the antiepilepsy drug vigabatrin (2004). https://doi.org/10.1074/jbc.M305884200 DOI: 10.1074/jbc.M305884200","model_system":"Pig liver GABA aminotransferase; inhibitor complexes and spectroscopy","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [storici-2004-gaba-at] Structures of gamma-aminobutyric acid (GABA) aminotransferase, a pyridoxal 5'-phosphate, and [2Fe-2S] cluster-containing enzyme, complexed with gamma-ethynyl-GABA and with the antiepilepsy drug vigabatrin (2004). https://doi.org/10.1074/jbc.M305884200 DOI: 10.1074/jbc.M305884200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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