{"id":"922decfb-82ba-5ee6-b6ac-56dec421f47e","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-hsd10-reaction","predicate":"oxidizes","statement":"HSD17B10 encodes the 2-methyl-3-hydroxybutyryl-CoA dehydrogenase step of isoleucine metabolism.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3c839ba1-14bb-5250-845b-711fe7f851e5","mechanism_event_label":"Another enzyme changes the intermediate before the final carbon split.","subject":{"id":"fa12d922-72dc-56c1-90bc-5dd448d97de4","slug":"hsd17b10","display_name":"Human hydroxysteroid dehydrogenase 10 / HSD17B10","entity_type_key":"protein"},"object":{"id":"38260a32-90f3-553d-b3cf-0b5392a5d649","slug":"2-methyl-3-hydroxybutyryl-coa","display_name":"2-Methyl-3-hydroxybutyryl-CoA","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"3c839ba1-14bb-5250-845b-711fe7f851e5","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-hsd10-reaction-event","event_type":"observed_relationship","label":"Another enzyme changes the intermediate before the final carbon split.","description":"HSD17B10 encodes the 2-methyl-3-hydroxybutyryl-CoA dehydrogenase step of isoleucine metabolism.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fa12d922-72dc-56c1-90bc-5dd448d97de4","slug":"hsd17b10","display_name":"Human hydroxysteroid dehydrogenase 10 / HSD17B10","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"38260a32-90f3-553d-b3cf-0b5392a5d649","slug":"2-methyl-3-hydroxybutyryl-coa","display_name":"2-Methyl-3-hydroxybutyryl-CoA","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c85b327e-f132-5d9a-8e4b-c2976fcc0373","slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2c795364-e2ff-5782-8270-81e153e92c9d","slug":"2-methylacetoacetyl-coa","display_name":"2-Methylacetoacetyl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"9fbf2e03-16a9-5ca7-965c-0bfc07ca24da","slug":"nadh","display_name":"NADH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Established biochemical function in a primary loss-of-function/rescue study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The protein also has essential functions beyond this reaction; metabolic activity alone does not explain its disease phenotype.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Isoleucine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Another enzyme changes the intermediate before the final carbon split.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A non-enzymatic function of 17beta-hydroxysteroid dehydrogenase type 10 is required for mitochondrial integrity and cell survival. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20077426/ · DOI 10.1002/emmm.200900055","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"538b2bc4-fd7a-5101-af5a-63a5602f3769","evidence_kind":"source_excerpt","locator":"Lines 210-216","start_line":210,"end_line":216,"excerpt":"## isoleucine-hsd10-reaction\nAnother enzyme changes the intermediate before the final carbon split.\nHSD17B10 encodes the 2-methyl-3-hydroxybutyryl-CoA dehydrogenase step of isoleucine metabolism.\nModel: Established biochemical function in a primary loss-of-function/rescue study.\nLimitations: The protein also has essential functions beyond this reaction; metabolic activity alone does not explain its disease phenotype.\nEvidence access: Primary abstract\nA non-enzymatic function of 17beta-hydroxysteroid dehydrogenase type 10 is required for mitochondrial integrity and cell survival. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20077426/ · DOI 10.1002/emmm.200900055","model_system":"Established biochemical function in a primary loss-of-function/rescue study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4b00f118-00fd-5cef-be97-13125eb958e9","stable_key":"import-09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2","title":"L-Isoleucine: transport, translation, catabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"2058ff0da379586994e7fda6ae0086c60fa3173542bfaf1fb3871ad3e09f9c6c","revision_id":"f32192d4-9913-5d35-acbc-cf90ff17995e","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}