{"id":"91b92725-ca83-5767-92ea-e526365ef139","stable_key":"b26c9d01-98fd-50d3-8f39-44db46961ee5:acetate-ffar3-on-l-cells","predicate":"is_expressed_in","statement":"The FFAR3 reporter was strongly expressed in all cholecystokinin, glucose-dependent insulinotropic peptide and secretin cells of the proximal small intestine, in all GLP-1, peptide YY and neurotensin cells of the distal small intestine, and in the large population of peptide YY and GLP-1 cells throughout the colon and rectum, and also in the neuronal cells of the submucosal and myenteric ganglia.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d4692edd-07eb-5ccc-86db-d572501a046b","mechanism_event_label":"The FFAR3 reporter marks nearly every hormone-making cell of the gut, and the gut nerves as well.","subject":{"id":"747bf692-56f7-59fd-98c2-e84a6f02dcb7","slug":"ffar3","display_name":"Human free fatty acid receptor 3 / FFAR3","entity_type_key":"protein"},"object":{"id":"ecbd32f4-d547-52ca-8f3f-3175bff73579","slug":"enteroendocrine-cell","display_name":"Enteroendocrine cell","entity_type_key":"cell_type"},"evidence_count":1,"mechanism_event":{"id":"d4692edd-07eb-5ccc-86db-d572501a046b","stable_key":"b26c9d01-98fd-50d3-8f39-44db46961ee5:acetate-ffar3-on-l-cells-event","event_type":"observed_intervention","label":"The FFAR3 reporter marks nearly every hormone-making cell of the gut, and the gut nerves as well.","description":"The FFAR3 reporter was strongly expressed in all cholecystokinin, glucose-dependent insulinotropic peptide and secretin cells of the proximal small intestine, in all GLP-1, peptide YY and neurotensin cells of the distal small intestine, and in the large population of peptide YY and GLP-1 cells throughout the colon and rectum, and also in the neuronal cells of the submucosal and myenteric ganglia.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e1ac8581-a2c5-5cfa-a9b0-9cc4f61be6e2","slug":"enteric-neuron","display_name":"Enteric neuron of the submucosal and myenteric ganglia","entity_type_key":"cell_type"},"role":"co_expressing_population","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4853afc6-9eaa-547f-91d7-c4dd90082351","slug":"glp-1","display_name":"Glucagon-like peptide 1 / GLP-1","entity_type_key":"peptide"},"role":"marker_peptide","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fd91b56b-3280-57b0-b2d0-61d5319616c0","slug":"pyy","display_name":"Peptide YY / PYY","entity_type_key":"peptide"},"role":"marker_peptide","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"747bf692-56f7-59fd-98c2-e84a6f02dcb7","slug":"ffar3","display_name":"Human free fatty acid receptor 3 / FFAR3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ecbd32f4-d547-52ca-8f3f-3175bff73579","slug":"enteroendocrine-cell","display_name":"Enteroendocrine cell","entity_type_key":"cell_type"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/acetate-research/23885020.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b834455e11c2b8fa3c4c19c5d01375815e46c3b0f8e25eb184f0eaa6a51c749e\", \"start_char\": 0, \"end_char\": 2144, \"text_sha256\": \"b834455e11c2b8fa3c4c19c5d01375815e46c3b0f8e25eb184f0eaa6a51c749e\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Transgenic monomeric red fluorescent protein reporter mice with FACS purification and quantitative PCR","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Cell-type resolved expression of FFAR2 and FFAR3 reporters, with receptor-specific synthetic agonists on colonic crypt cultures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reporter expression is not the same as receptor protein. The FFAR2 result here is weaker in enteroendocrine cells than the rat immunohistochemistry in this collection reports.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Acetic acid research collection; topical membership is not evidence of a direct clinical effect, and the ingested acid is recorded separately from the circulating acetate anion.","comparator":null,"unit":null,"notes":"","entity":{"slug":"acetic-acid","display_name":"Acetic acid","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The FFAR3 reporter marks nearly every hormone-making cell of the gut, and the gut nerves as well.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[acetate-p23885020] GPR41/FFAR3 and GPR43/FFAR2 as cosensors for short-chain fatty acids in enteroendocrine cells vs FFAR3 in enteric neurons and FFAR2 in enteric leukocytes. (2013). https://pubmed.ncbi.nlm.nih.gov/23885020/ DOI: 10.1210/en.2013-1142","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Whole gastrointestinal tract","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fca1d754-24c0-5e19-a725-0cdcf31c935d","evidence_kind":"source_excerpt","locator":"Lines 290-301","start_line":290,"end_line":301,"excerpt":"### acetate-ffar3-on-l-cells\nThe FFAR3 reporter was strongly expressed in all cholecystokinin, glucose-dependent insulinotropic peptide and secretin cells of the proximal small intestine, in all GLP-1, peptide YY and neurotensin cells of the distal small intestine, and in the large population of peptide YY and GLP-1 cells throughout the colon and rectum, and also in the neuronal cells of the submucosal and myenteric ganglia.\nCondition category: normal\nnutrient_topic: Acetic acid research collection; topical membership is not evidence of a direct clinical effect, and the ingested acid is recorded separately from the circulating acetate anion.\nplain_language: The FFAR3 reporter marks nearly every hormone-making cell of the gut, and the gut nerves as well.\norganism: Mouse\ntissue_or_cell_type: Whole gastrointestinal tract\nexperimental_model: Transgenic monomeric red fluorescent protein reporter mice with FACS purification and quantitative PCR\nlimitations: Reporter expression is not the same as receptor protein. The FFAR2 result here is weaker in enteroendocrine cells than the rat immunohistochemistry in this collection reports.\nexposure: Cell-type resolved expression of FFAR2 and FFAR3 reporters, with receptor-specific synthetic agonists on colonic crypt cultures\nevidence_span: {\"source_cache\": \"artifacts/acetate-research/23885020.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b834455e11c2b8fa3c4c19c5d01375815e46c3b0f8e25eb184f0eaa6a51c749e\", \"start_char\": 0, \"end_char\": 2144, \"text_sha256\": \"b834455e11c2b8fa3c4c19c5d01375815e46c3b0f8e25eb184f0eaa6a51c749e\"}\n[acetate-p23885020] GPR41/FFAR3 and GPR43/FFAR2 as cosensors for short-chain fatty acids in enteroendocrine cells vs FFAR3 in enteric neurons and FFAR2 in enteric leukocytes. 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