{"id":"91a2d379-3657-5387-9a2f-40820573845f","stable_key":"773f772e-bf8e-5e52-a2a2-6dd694d67f5d:bromelain-cd25-cleavage","predicate":"cleaves_from_cell_surface","statement":"Bromelain reduced surface CD25 on activated human CD4 T cells while soluble CD25 increased, supporting proteolytic shedding.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"e125a678-bb4c-5766-8516-0dd29ab1d306","mechanism_event_label":"Bromelain reduced surface CD25 on activated human CD4 T cells while soluble CD25 increased, supporting proteolytic shedding.","subject":{"id":"6a3990e6-85af-58c7-8087-8a251db842fa","slug":"bromelain","display_name":"Bromelain","entity_type_key":"peptide"},"object":{"id":"4d639537-19f6-51d0-984d-c58db2e3f1d2","slug":"human-cd25","display_name":"Human interleukin-2 receptor alpha / CD25","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"e125a678-bb4c-5766-8516-0dd29ab1d306","stable_key":"773f772e-bf8e-5e52-a2a2-6dd694d67f5d:bromelain-cd25-cleavage-event","event_type":"observed_relationship","label":"Bromelain reduced surface CD25 on activated human CD4 T cells while soluble CD25 increased, supporting proteolytic shedding.","description":"Bromelain reduced surface CD25 on activated human CD4 T cells while soluble CD25 increased, supporting proteolytic shedding.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6a3990e6-85af-58c7-8087-8a251db842fa","slug":"bromelain","display_name":"Bromelain","entity_type_key":"peptide"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4d639537-19f6-51d0-984d-c58db2e3f1d2","slug":"human-cd25","display_name":"Human interleukin-2 receptor alpha / CD25","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Bromelain 25-100 micrograms/mL","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Up to 8 hours","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Anti-CD3-activated human CD4 T cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Receptor shedding in activated cells does not establish the net immune effect after oral use.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Bromelain chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"bromelain","display_name":"Bromelain","entity_type_key":"peptide"}},{"dimension":"organism","value_text":"Anti-CD3-activated human CD4 T cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Bromelain reduced surface CD25 on activated human CD4 T cells while soluble CD25 increased, supporting proteolytic shedding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Bromelain treatment reduces CD25 expression on activated CD4+ T cells in vitro. (2009). https://pubmed.ncbi.nlm.nih.gov/19162239/ DOI: 10.1016/j.intimp.2008.12.012","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Surface and soluble CD25","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3943e3db-1cfd-5fd6-8374-9f608a7bdf54","evidence_kind":"source_excerpt","locator":"Lines 55-64","start_line":55,"end_line":64,"excerpt":"## bromelain-cd25-cleavage\nBromelain reduced surface CD25 on activated human CD4 T cells while soluble CD25 increased, supporting proteolytic shedding.\nModel/species: Anti-CD3-activated human CD4 T cells\nTissue/system: Surface and soluble CD25\nExposure: Bromelain 25-100 micrograms/mL\nRoute: In vitro\nDuration: Up to 8 hours\nLimits: Receptor shedding in activated cells does not establish the net immune effect after oral use.\nPrimary reference: Bromelain treatment reduces CD25 expression on activated CD4+ T cells in vitro. (2009). https://pubmed.ncbi.nlm.nih.gov/19162239/ DOI: 10.1016/j.intimp.2008.12.012\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Anti-CD3-activated human CD4 T cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9d363b4e-f438-5664-9d74-99c2e359391d","stable_key":"import-773f772e-bf8e-5e52-a2a2-6dd694d67f5d","title":"Bromelain: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"b328dbb8082bea612bb2c40d6ff41fa670f6e62f714bd014c90627fcab86e95b","revision_id":"1c10d22e-ecdf-56ef-b820-bb21182a9577","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}