{"id":"91755899-0371-5d16-80bf-c20ca34b9f4d","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-mouse-nrf2-loss","predicate":"knockout_removes_distinct_response","statement":"The distinct luteolin-associated liver responses were absent in Nrf2-knockout mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1176291f-5882-5b12-bdd5-7b88a7c38d91","mechanism_event_label":"Removing the regulator changed the response to the compound.","subject":{"id":"95319607-3946-51b3-8235-400ec3223692","slug":"mouse-nfe2l2","display_name":"Mouse Nrf2 / Nfe2l2","entity_type_key":"protein"},"object":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"1176291f-5882-5b12-bdd5-7b88a7c38d91","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-mouse-nrf2-loss-event","event_type":"observed_relationship","label":"Removing the regulator changed the response to the compound.","description":"The distinct luteolin-associated liver responses were absent in Nrf2-knockout mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"95319607-3946-51b3-8235-400ec3223692","slug":"mouse-nfe2l2","display_name":"Mouse Nrf2 / Nfe2l2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Wild-type versus Nrf2-null mice.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Knockout context does not define a dietary deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Removing the regulator changed the response to the compound.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Luteolin inhibits the Nrf2 signaling pathway and tumor growth in vivo. · 2014 · https://pubmed.ncbi.nlm.nih.gov/24747074/ · DOI 10.1016/j.bbrc.2014.04.039","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"63cc775b-3c52-593b-80c5-19fd6948d416","evidence_kind":"source_excerpt","locator":"Lines 460-466","start_line":460,"end_line":466,"excerpt":"## luteolin-mouse-nrf2-loss\nRemoving the regulator changed the response to the compound.\nThe distinct luteolin-associated liver responses were absent in Nrf2-knockout mice.\nModel: Wild-type versus Nrf2-null mice.\nLimitations: Knockout context does not define a dietary deficiency.\nEvidence access: Primary abstract\nLuteolin inhibits the Nrf2 signaling pathway and tumor growth in vivo. · 2014 · https://pubmed.ncbi.nlm.nih.gov/24747074/ · DOI 10.1016/j.bbrc.2014.04.039","model_system":"Wild-type versus Nrf2-null mice.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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