{"id":"91646b5e-f116-5a70-84e4-2620bc849504","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-gsta1-inhibition","predicate":"reversibly_inhibits","statement":"Fisetin reversibly inhibited purified human GSTA1-1 with IC50 1.2 +/- 0.1 micromolar; inhibition was mixed toward glutathione and noncompetitive toward CDNB.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"a2105a3a-2fe4-5e21-a10a-fe4778f236b0","mechanism_event_label":"A compound that supports a glutathione pool can also inhibit an enzyme using it.","subject":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"object":{"id":"35e312f9-e82c-5434-9357-1cfa61fe85e8","slug":"gsta1","display_name":"Human glutathione S-transferase A1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a2105a3a-2fe4-5e21-a10a-fe4778f236b0","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-gsta1-inhibition-event","event_type":"observed_relationship","label":"A compound that supports a glutathione pool can also inhibit an enzyme using it.","description":"Fisetin reversibly inhibited purified human GSTA1-1 with IC50 1.2 +/- 0.1 micromolar; inhibition was mixed toward glutathione and noncompetitive toward CDNB.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"35e312f9-e82c-5434-9357-1cfa61fe85e8","slug":"gsta1","display_name":"Human glutathione S-transferase A1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human enzyme kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"CDNB probe assay, not proven human chemosensitization or global detoxification failure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A compound that supports a glutathione pool can also inhibit an enzyme using it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The Interaction of the Flavonoid Fisetin with Human Glutathione Transferase A1-1. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33806779/ · DOI 10.3390/metabo11030190","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a0197bc2-c522-5941-b95b-c00190dbe34f","evidence_kind":"source_excerpt","locator":"Lines 88-94","start_line":88,"end_line":94,"excerpt":"## fisetin-gsta1-inhibition\nA compound that supports a glutathione pool can also inhibit an enzyme using it.\nFisetin reversibly inhibited purified human GSTA1-1 with IC50 1.2 +/- 0.1 micromolar; inhibition was mixed toward glutathione and noncompetitive toward CDNB.\nModel: Purified human enzyme kinetics.\nLimitations: CDNB probe assay, not proven human chemosensitization or global detoxification failure.\nEvidence access: Primary abstract\nThe Interaction of the Flavonoid Fisetin with Human Glutathione Transferase A1-1. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33806779/ · DOI 10.3390/metabo11030190","model_system":"Purified human enzyme kinetics.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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