{"id":"915a8d21-5c1a-50a0-9e7a-93c04345edb2","stable_key":"e3f6ce02-0bd7-5103-acc1-68503ab6751b:rat-lipoic-metal-null","predicate":"no_detected_change_in_recorded_comparison","statement":"R-lipoic-acid-containing treatment did not lower total brain iron or copper in the reported old-rat study.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"b17f4a80-ffa0-542b-9294-6c118bec602a","mechanism_event_label":"R-lipoic-acid-containing treatment did not lower total brain iron or copper in the reported old-rat study.","subject":{"id":"8eeb8f85-a924-5122-9399-a757b83c668c","slug":"r-lipoic-acid","display_name":"Free R-alpha-lipoic acid","entity_type_key":"small_molecule"},"object":{"id":"8dec072a-be7f-59c3-bb6c-5ae8cfc0ebc8","slug":"rat-aged-brain-metal-content","display_name":"Aged-rat brain total iron and copper","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b17f4a80-ffa0-542b-9294-6c118bec602a","stable_key":"e3f6ce02-0bd7-5103-acc1-68503ab6751b:rat-lipoic-metal-null-event","event_type":"experimental_observation","label":"R-lipoic-acid-containing treatment did not lower total brain iron or copper in the reported old-rat study.","description":"### rat-lipoic-metal-null\n\nR-lipoic-acid-containing treatment did not lower total brain iron or copper in the reported old-rat study.\nA null total-metal result is not a measurement of labile-metal pools.\nModel and exposure: Seven-week aged-rat intervention; total-metal measurement.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Age-associated mitochondrial oxidative decay: improvement of carnitine acetyltransferase substrate-binding affinity and activity in brain by feeding old rats acetyl-L- carnitine and/or R-alpha -lipoic acid. | 2002 | https://pubmed.ncbi.nlm.nih.gov/11854488/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC122287/ | https://doi.org/10.1073/pnas.261709098 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.\nReference: Correction | 2002 | https://pmc.ncbi.nlm.nih.gov/articles/PMC124428/ | Access: Full primary correction read: adds Juvenon financial-interest disclosures, not a changed biochemical result.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8eeb8f85-a924-5122-9399-a757b83c668c","slug":"r-lipoic-acid","display_name":"Free R-alpha-lipoic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"R-lipoic acid alone or with ALCAR","sequence_order":0,"notes":""},{"entity":{"id":"8dec072a-be7f-59c3-bb6c-5ae8cfc0ebc8","slug":"rat-aged-brain-metal-content","display_name":"Aged-rat brain total iron and copper","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"no_detected_change","sequence_order":1,"notes":""},{"entity":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"role":"combination-arm exposure","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"role":"measured element","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"9f0afdde-1ec1-5c8a-bb5e-f3b2b75f67f6","slug":"copper","display_name":"Copper","entity_type_key":"nutrient_element"},"role":"measured element","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.; Full primary correction read: adds Juvenon financial-interest disclosures, not a changed biochemical result.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"R-lipoic acid alone or with ALCAR\", \"comparator\": \"Untreated aged rats\", \"endpoint\": \"R-lipoic-acid-containing treatment did not lower total brain iron or copper in the reported old-rat study.\", \"effect_direction\": \"no_detected_change\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Seven-week aged-rat intervention; total-metal measurement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived curation with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Acetyl-L-carnitine collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"R-lipoic-acid-containing treatment did not lower total brain iron or copper in the reported old-rat study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Age-associated mitochondrial oxidative decay: improvement of carnitine acetyltransferase substrate-binding affinity and activity in brain by feeding old rats acetyl-L- carnitine and/or R-alpha -lipoic acid. | https://pubmed.ncbi.nlm.nih.gov/11854488/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC122287/ | https://doi.org/10.1073/pnas.261709098","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Correction | https://pmc.ncbi.nlm.nih.gov/articles/PMC124428/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference rat-lipoic-metal-null; A null total-metal result is not a measurement of labile-metal pools.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9de430c4-79d3-58ab-8a9f-f0ea45bf9622","evidence_kind":"source_excerpt","locator":"Lines 462-469","start_line":462,"end_line":469,"excerpt":"### rat-lipoic-metal-null\n\nR-lipoic-acid-containing treatment did not lower total brain iron or copper in the reported old-rat study.\nA null total-metal result is not a measurement of labile-metal pools.\nModel and exposure: Seven-week aged-rat intervention; total-metal measurement.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Age-associated mitochondrial oxidative decay: improvement of carnitine acetyltransferase substrate-binding affinity and activity in brain by feeding old rats acetyl-L- carnitine and/or R-alpha -lipoic acid. | 2002 | https://pubmed.ncbi.nlm.nih.gov/11854488/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC122287/ | https://doi.org/10.1073/pnas.261709098 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.\nReference: Correction | 2002 | https://pmc.ncbi.nlm.nih.gov/articles/PMC124428/ | Access: Full primary correction read: adds Juvenon financial-interest disclosures, not a changed biochemical result.","model_system":"Seven-week aged-rat intervention; total-metal measurement.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2588f904-245a-597a-8a12-65118013fb90","stable_key":"import-e3f6ce02-0bd7-5103-acc1-68503ab6751b","title":"Acetyl-L-carnitine: detailed mechanisms of action (reviewed 6 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original review of primary studies and clearly identified official records. Exact primary access is stated; no independent replication is claimed.","file_path":"","sha256":"84cc82d6fbb0fd5d73c4ce779e9f88e7f0184ba2d29b18ad0f153815380f2975","revision_id":"dac7a950-dbe5-5c7a-9c70-2ea711d5c71a","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}