{"id":"90deb3ea-9882-5a57-ba4d-d973c48b743a","stable_key":"e3f6ce02-0bd7-5103-acc1-68503ab6751b:hcc-fatty-acid-pool-null","predicate":"no_detected_change_in_recorded_comparison","statement":"ALCAR supplementation did not change measured fatty-acid abundance across the tested HCC genotypes.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"41aa7f50-d890-5a51-9758-54046895f515","mechanism_event_label":"ALCAR supplementation did not change measured fatty-acid abundance across the tested HCC genotypes.","subject":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"object":{"id":"4086302c-d02b-5f72-b9fa-2309bbc5115c","slug":"mouse-hcc-fatty-acid-abundance","display_name":"Mouse HCC fatty-acid abundance","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"41aa7f50-d890-5a51-9758-54046895f515","stable_key":"e3f6ce02-0bd7-5103-acc1-68503ab6751b:hcc-fatty-acid-pool-null-event","event_type":"experimental_observation","label":"ALCAR supplementation did not change measured fatty-acid abundance across the tested HCC genotypes.","description":"### hcc-fatty-acid-pool-null\n\nALCAR supplementation did not change measured fatty-acid abundance across the tested HCC genotypes.\nA change in isotope labeling or synthesis contribution is not necessarily an increase in total fatty-acid abundance.\nModel and exposure: Mouse HCC, wild-type/ACLY-null/ACLY-ACSS2-null; supplementary S7H–I described in Results.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Acetylcarnitine shuttling links mitochondrial metabolism to histone acetylation and lipogenesis. | 2023 | https://pubmed.ncbi.nlm.nih.gov/37134161/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC10156126/ | https://doi.org/10.1126/sciadv.adf0115 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"ALCAR supplementation","sequence_order":0,"notes":""},{"entity":{"id":"4086302c-d02b-5f72-b9fa-2309bbc5115c","slug":"mouse-hcc-fatty-acid-abundance","display_name":"Mouse HCC fatty-acid abundance","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"no_detected_change","sequence_order":1,"notes":""},{"entity":{"id":"283ce4c4-3ba6-5f26-93df-9939ef46d5fe","slug":"mouse-acly","display_name":"Mouse ATP-citrate lyase / Acly","entity_type_key":"protein"},"role":"genotype context","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"af781f8d-c5d0-575e-a9e0-09e4b8d5165a","slug":"mouse-acss2","display_name":"Mouse acyl-CoA synthetase short-chain family member 2 / Acss2","entity_type_key":"protein"},"role":"genotype context","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"ALCAR supplementation\", \"comparator\": \"Corresponding unsupplemented genotype\", \"endpoint\": \"ALCAR supplementation did not change measured fatty-acid abundance across the tested HCC genotypes.\", \"effect_direction\": \"no_detected_change\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Mouse HCC, wild-type/ACLY-null/ACLY-ACSS2-null; supplementary S7H–I described in Results.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived curation with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Acetyl-L-carnitine collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"ALCAR supplementation did not change measured fatty-acid abundance across the tested HCC genotypes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Acetylcarnitine shuttling links mitochondrial metabolism to histone acetylation and lipogenesis. | https://pubmed.ncbi.nlm.nih.gov/37134161/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC10156126/ | https://doi.org/10.1126/sciadv.adf0115","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference hcc-fatty-acid-pool-null; A change in isotope labeling or synthesis contribution is not necessarily an increase in total fatty-acid abundance.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0aea402e-671c-56e0-a6d4-5fa3baca86e2","evidence_kind":"source_excerpt","locator":"Lines 276-282","start_line":276,"end_line":282,"excerpt":"### hcc-fatty-acid-pool-null\n\nALCAR supplementation did not change measured fatty-acid abundance across the tested HCC genotypes.\nA change in isotope labeling or synthesis contribution is not necessarily an increase in total fatty-acid abundance.\nModel and exposure: Mouse HCC, wild-type/ACLY-null/ACLY-ACSS2-null; supplementary S7H–I described in Results.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Acetylcarnitine shuttling links mitochondrial metabolism to histone acetylation and lipogenesis. | 2023 | https://pubmed.ncbi.nlm.nih.gov/37134161/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC10156126/ | https://doi.org/10.1126/sciadv.adf0115 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"Mouse HCC, wild-type/ACLY-null/ACLY-ACSS2-null; supplementary S7H–I described in Results.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2588f904-245a-597a-8a12-65118013fb90","stable_key":"import-e3f6ce02-0bd7-5103-acc1-68503ab6751b","title":"Acetyl-L-carnitine: detailed mechanisms of action (reviewed 6 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original review of primary studies and clearly identified official records. Exact primary access is stated; no independent replication is claimed.","file_path":"","sha256":"84cc82d6fbb0fd5d73c4ce779e9f88e7f0184ba2d29b18ad0f153815380f2975","revision_id":"dac7a950-dbe5-5c7a-9c70-2ea711d5c71a","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}