{"id":"90d6eab2-e5f3-527c-a110-c1d58d3fcca7","stable_key":"b3c3f45a-50fb-5b79-98d7-5e5228df00db:cold-rbm3-protein-synthesis","predicate":"increases","statement":"Cells overexpressing Rbm3 showed a three-fold increase in protein synthesis at both 37 and 32 degrees C, a fraction of Rbm3 associated with 60S ribosomal subunits in an RNA-independent manner, and a microRNA-containing complex was less abundant in those cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"5ed2f60c-9ac3-596b-b030-eb9fdaaaa078","mechanism_event_label":"The cold-induced protein makes cells build more protein, and it sits on the ribosome to do it.","subject":{"id":"60f85d64-9328-5875-a27f-a5dd85087633","slug":"rbm3","display_name":"RNA-binding motif protein 3 / RBM3 cold-shock protein","entity_type_key":"protein"},"object":{"id":"383297b4-a416-5bd6-9f42-11af1aca08f2","slug":"global-protein-synthesis","display_name":"Global cellular protein synthesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5ed2f60c-9ac3-596b-b030-eb9fdaaaa078","stable_key":"b3c3f45a-50fb-5b79-98d7-5e5228df00db:cold-rbm3-protein-synthesis-event","event_type":"biochemical_relationship","label":"The cold-induced protein makes cells build more protein, and it sits on the ribosome to do it.","description":"Cells overexpressing Rbm3 showed a three-fold increase in protein synthesis at both 37 and 32 degrees C, a fraction of Rbm3 associated with 60S ribosomal subunits in an RNA-independent manner, and a microRNA-containing complex was less abundant in those cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"91907eef-798d-5ea5-8e1a-a7f8a810c05d","slug":"ribosomal-60s-subunit","display_name":"The 60S ribosomal subunit","entity_type_key":"protein_complex"},"role":"binding_partner","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"29ae8b9a-8b88-5aab-b211-fec1ca89969d","slug":"microrna-levels","display_name":"Cellular microRNA levels","entity_type_key":"cellular_process"},"role":"altered_measure","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"60f85d64-9328-5875-a27f-a5dd85087633","slug":"rbm3","display_name":"RNA-binding motif protein 3 / RBM3 cold-shock protein","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"383297b4-a416-5bd6-9f42-11af1aca08f2","slug":"global-protein-synthesis","display_name":"Global cellular protein synthesis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/cold-research/15684048.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"662a7c449d01ae63eabee803791ad8154faa37eb62f60ccf58aabc3bd355d7e5\", \"start_char\": 0, \"end_char\": 1694, \"text_sha256\": \"662a7c449d01ae63eabee803791ad8154faa37eb62f60ccf58aabc3bd355d7e5\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse neuroblastoma cells overexpressing Rbm3 as a c-Myc fusion protein at 37 and 32 degrees C","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Mild hypothermia at 32 degrees C with Rbm3 overexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A cell-culture overexpression study. The microRNA explanation is offered as a possibility by the authors rather than demonstrated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cold water immersion research collection; topical membership is not evidence of a direct clinical effect, and a therapeutic exposure is not a dietary intake.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cold-water-immersion","display_name":"Cold water immersion","entity_type_key":"drug"}},{"dimension":"organism","value_text":"Mouse cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The cold-induced protein makes cells build more protein, and it sits on the ribosome to do it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[cold-p15684048] Cold stress-induced protein Rbm3 binds 60S ribosomal subunits, alters microRNA levels, and enhances global protein synthesis. (2005). https://pubmed.ncbi.nlm.nih.gov/15684048/ DOI: 10.1073/pnas.0409764102","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Neuroblastoma cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"74cb1e5f-54cf-50dd-b4ef-d3e2ae8c7f08","evidence_kind":"source_excerpt","locator":"Lines 897-908","start_line":897,"end_line":908,"excerpt":"### cold-rbm3-protein-synthesis\nCells overexpressing Rbm3 showed a three-fold increase in protein synthesis at both 37 and 32 degrees C, a fraction of Rbm3 associated with 60S ribosomal subunits in an RNA-independent manner, and a microRNA-containing complex was less abundant in those cells.\nCondition category: normal\nnutrient_topic: Cold water immersion research collection; topical membership is not evidence of a direct clinical effect, and a therapeutic exposure is not a dietary intake.\nplain_language: The cold-induced protein makes cells build more protein, and it sits on the ribosome to do it.\norganism: Mouse cells\ntissue_or_cell_type: Neuroblastoma cells\nexperimental_model: Mouse neuroblastoma cells overexpressing Rbm3 as a c-Myc fusion protein at 37 and 32 degrees C\nlimitations: A cell-culture overexpression study. The microRNA explanation is offered as a possibility by the authors rather than demonstrated.\nexposure: Mild hypothermia at 32 degrees C with Rbm3 overexpression\nevidence_span: {\"source_cache\": \"artifacts/cold-research/15684048.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"662a7c449d01ae63eabee803791ad8154faa37eb62f60ccf58aabc3bd355d7e5\", \"start_char\": 0, \"end_char\": 1694, \"text_sha256\": \"662a7c449d01ae63eabee803791ad8154faa37eb62f60ccf58aabc3bd355d7e5\"}\n[cold-p15684048] Cold stress-induced protein Rbm3 binds 60S ribosomal subunits, alters microRNA levels, and enhances global protein synthesis. (2005). https://pubmed.ncbi.nlm.nih.gov/15684048/ DOI: 10.1073/pnas.0409764102","model_system":"Mouse neuroblastoma cells overexpressing Rbm3 as a c-Myc fusion protein at 37 and 32 degrees C","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [cold-p15684048] Cold stress-induced protein Rbm3 binds 60S ribosomal subunits, alters microRNA levels, and enhances global protein synthesis. (2005). https://pubmed.ncbi.nlm.nih.gov/15684048/ DOI: 10.1073/pnas.0409764102","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"feeccadc-1392-573e-a0b5-0939ee5de6e2","stable_key":"import-b3c3f45a-50fb-5b79-98d7-5e5228df00db","title":"Cold water immersion: cold sensing, heat production, the catecholamine axis and what repeated exposure changes (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"557f074d372c49c502c34e648651aff7ebf2c8816305d2d2f08e338a7e96a5f0","revision_id":"ecc19e8d-40db-5b5d-b98e-0507f645976c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}